Application | Comment | Organism |
---|---|---|
medicine | may have implications for the diagnosis of hypophosphatasia and treatment of bone mineralization abnormalities such as osteomalacia and pathological soft-tissue ossification, a process clinically significant in atheroscleosis and heart failure | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TOP10 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
O-Phosphoethanolamine | pH 6.7, 37°C, 2 mM Mg2+ | Homo sapiens | |
0.0114 | - |
phosphocholine | pH 6.7, 37°C, 2 mM Mg2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex | Homo sapiens | |
Mg2+ | high specific Mg2+-dependence, optimum concentration: 2 mM MgCl2 | Homo sapiens | |
Mn2+ | stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex | Homo sapiens | |
additional information | not activated by Ca2+ or Zn2+ | Homo sapiens | |
Ni2+ | stimulates to a lesser extend than Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phosphoethanolamine + H2O | Homo sapiens | likely a natural substrate, phosphoethanolamine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization | ethanolamine + phosphate | - |
? | |
phosphocholine + H2O | Homo sapiens | likely a natural substrate, phosphocholine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization | choline + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PHOSPHO1 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-phosphoethanolamine + H2O = ethanolamine + phosphate | requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine | Homo sapiens | |
phosphocholine + H2O = choline + phosphate | requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SAOS-2 cell | osteoblast-like cells | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0176 | - |
pH 7.2, 37°C, hydrolysis of pyridoxal 5-phosphate | Homo sapiens |
0.0396 | - |
pH 7.2, 37°C, hydrolysis of beta-glycerol phosphate | Homo sapiens |
0.0645 | - |
pH 7.2, 37°C, hydrolysis of p-nitrophenyl phosphate | Homo sapiens |
0.0748 | - |
pH 7.2, 37°C, hydrolysis of ribose 5-phosphate | Homo sapiens |
2.98 | - |
pH 7.2, 37°C, hydrolysis of phosphocholine | Homo sapiens |
4.6 | - |
pH 7.2, 37°C, hydrolysis of phosphoethanolamine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glycerol phosphate + H2O | poor substrate | Homo sapiens | glycerol + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | poor substrate | Homo sapiens | D-ribose + phosphate | - |
? | |
additional information | not: diphosphate, phospho-L-serine, glycone phosphate, fructose 6-phosphate, phospho-L-tyrosine, ATP | Homo sapiens | ? | - |
? | |
O-phosphoethanolamine + H2O | likely a natural substrate, phosphoethanolamine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization | Homo sapiens | ethanolamine + phosphate | - |
? | |
O-phosphoethanolamine + H2O | PHOSPHO1 exhibits high specific activities toward phosphoethanolamine and phosphocholine, phosphoethanolamine is hydrolyzed 1.5times faster than phosphocholine | Homo sapiens | ethanolamine + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | poor substrate | Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
phosphocholine + H2O | likely a natural substrate, phosphocholine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization | Homo sapiens | choline + phosphate | - |
? | |
phosphocholine + H2O | PHOSPHO1 exhibits high specific activities toward phosphoethanolamine and phosphocholine, phosphocholine is hydrolyzed 1.5times slower than phosphoethanolamine | Homo sapiens | choline + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | poor substrate | Homo sapiens | pyridoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
3X11A | - |
Homo sapiens |
More | PHOSPHO1 is a member of the haloacid dehalogenase superfamily of Mg2+-dependent hydrolases | Homo sapiens |
PHOSPHO1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.98 | - |
phosphocholine | pH 6.7, 37°C, 2 mM Mg2+ | Homo sapiens | |
2.27 | - |
O-Phosphoethanolamine | pH 6.7, 37°C, 2 mM Mg2+ | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
around | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | above 70% of maximum activity between pH 6 and 7.2, activity with phosphocholine declines significantly above pH 7.2, activity with phosphoethanolamine declines above pH 7.5 | Homo sapiens |