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Literature summary for 3.1.3.74 extracted from
- Cloning of the pyridoxine 5-phosphate phosphatase gene (pdxP) and vitamin B(6) production in pdxP recombinant Sinorhizobium meliloti (2008), Biosci. Biotechnol. Biochem., 72, 421-427.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | possibility of development of the pyridoxine production process by a metabolic engineering approach. In the future, overexpression of the pdxP gene in combination with another pyridoxine gene will be necessary to develop even more efficient processes for production of vitamin B6 | Sinorhizobium meliloti |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pCRII-TOPO vector, 0.86 kb SmaI fragment from TOPOpdxP ligated into the SmaI site of pKK223-3, overexpression in Escherichia coli JM109. Overexpression in Sinorhizobium meliloti IFO 14782 recombinant with pVKPtacpdxP | Sinorhizobium meliloti |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26470 | - |
sequence analysis | Sinorhizobium meliloti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | A7BK78 | IFO 14782 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Sinorhizobium meliloti IFO 14782/pVKPtacpdxP shows PNP phosphatase activity 3.5times higher than that of the parent strain, when 2 mM pyridoxine 5'-phosphate is used as substrate. When pyridoxamine 5'-phosphate is used as substrate, the phosphatase activity of Sinorhizobium meliloti IFO 14782/pVKPtacpdxP is as low as that of the parent | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PNP phosphatase does not act on pyridoxamine 5'-phosphate | Sinorhizobium meliloti | ? | - |
? |  |
| pyridoxine 5'-phosphate + H2O | - |
Sinorhizobium meliloti | pyridoxine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNP phosphatase | - |
Sinorhizobium meliloti |
| pyridoxine 5'-phosphate phosphatase | - |
Sinorhizobium meliloti |
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