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Literature summary for 3.1.3.74 extracted from
- Purification and characterization of pyridoxine 5'-phosphate phosphatase from Sinorhizobium meliloti (2005), Biosci. Biotechnol. Biochem., 69, 2277-2284.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodacetate | 52% inhibition at 5 mM | Sinorhizobium meliloti |  |
| KH2PO4 | 56% inhibition at 5 mM | Sinorhizobium meliloti | |
| levamisole | 26% inhibition at 5 mM | Sinorhizobium meliloti | |
| N-ethylmaleimide | 46% inhibition at 5 mM | Sinorhizobium meliloti | |
| NaF | 24% inhibition at 5 mM | Sinorhizobium meliloti | |
| Sodium molybdate | 79% inhibition at 5 mM | Sinorhizobium meliloti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.385 | - |
pyridoxal-5'-phosphate | 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti | |
| 0.385 | - |
pyridoxine-5'-phosphate | 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Sinorhizobium meliloti | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | either Mg2+ or Mn2+ required, highest activity at 2 mM | Sinorhizobium meliloti | |
| Mn2+ | either Mg2+ or Mn2+ required, highest activity at 2 mM | Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Sinorhizobium meliloti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5 chromatography steps to homogeneity | Sinorhizobium meliloti |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Sinorhizobium meliloti | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42 | - |
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti |
| 105 | - |
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxal-5'-phosphate + H2O | - |
Sinorhizobium meliloti | pyridoxal + phosphate | - |
? | |
| pyridoxine-5'-phosphate + H2O | - |
Sinorhizobium meliloti | pyridoxine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Sinorhizobium meliloti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNP phosphatase | - |
Sinorhizobium meliloti |
| pyridoxal-5'-phosphate phosphatase | - |
Sinorhizobium meliloti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Sinorhizobium meliloti |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Sinorhizobium meliloti |
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