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Literature summary for 3.1.3.74 extracted from
- Kinetic analysis and chemical modification of vitamin B6 phosphatase from human erythrocytes (1994), J. Biol. Chem., 269, 7163-7168.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Homo sapiens |  |
| diethyldicarbonate | inactivates by reacting with a group with a pKalpha of 6.7, kinetics, pyridoxine 5-phosphate protects, 100 mM neutral hydroxylamine partially reactivates | Homo sapiens | |
| N-(5'-phospho-4'-pyridoxyl)ethanolamine | 0.05 mM, 12% inhibition | Homo sapiens | |
| N-(5'-phospho-4'-pyridoxyl)glycine | 0.05 mM, 32% inhibition | Homo sapiens | |
| N-(5'-phospho-4'-pyridoxyl)phenylalanine | 0.05 mM, 51% inhibition | Homo sapiens | |
| Phenylglyoxal | the incorporation of 1 mol per subunit inactivates, pyridoxal 5-phosphate protects, kinetics | Homo sapiens | |
| pyridoxine 5'-phosphate | 0.05 mM, 45% inhibition | Homo sapiens | |
| Tetranitromethane | inactivates in a time-dependent manner, 10 mM, 70% inhibition in the absence of pyridoxal 5-phosphate and 30% in the presence of 0.15 mM pyridoxal 5-phosphate | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data, effect of pH on Km for 4-pyridoxic acid 5-phosphate, pyridoxal 5-phosphate, pyridoxine 5-phosphate and pyridoxamine 5-phosphate | Homo sapiens | |
| 0.00112 | - |
4-pyridoxic acid 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
| 0.00129 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
| 0.00824 | - |
N-(5'-phospho-4'-pyridoxyl)phenylalanine | pH 7.4, 37°C | Homo sapiens | |
| 0.0106 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
| 0.0143 | - |
N-(5'-phospho-4'-pyridoxyl)glycine | pH 7.4, 37°C | Homo sapiens | |
| 0.0547 | - |
pyridoxamine 5'-phosphate | pH 7.4, 37°C | Homo sapiens | |
| 0.0635 | - |
N-(5'-phospho-4'-pyridoxyl)benzylamine | pH 7.4, 37°C | Homo sapiens | |
| 0.0786 | - |
N-(5'-phospho-4'-pyridoxyl)ethanolamine | pH 7.4, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
- |
Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 168000fold | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
pH 7.4, 37°C | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, storage at concentrations of 0.3 mg/ml or greater, 30 mM phosphate, at least 6 months, stable | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-pyridoxic acid 5'-phosphate + H2O | highest catalytic efficiency with 4-pyridoxic acid 5-phosphate and pyridoxal 5-phosphate | Homo sapiens | 4-pyridoxic acid + phosphate | - |
? | |
| additional information | specificity and active site properties, enzyme also catalyzes the dephosphorylation of 4-secondary amine derivatives of vitamin B6 phosphate, enzyme has the greatest catalytic efficiency with substrates that contain a negatively charged group on the 4-position of the pyridine ring, one or two positively charged groups at the active site of enzyme interacts with the substrates phosphate ester and 4-substituent, Arg and His residues are at or near the active site and may play roles in substrate binding and/or catalysis, very low activity with p-nitrophenylphosphate | Homo sapiens | ? | - |
? | |
| N-(5'-phospho-4'-pyridoxyl)benzylamine + H2O | - |
Homo sapiens | 4'-pyridoxylbenzylamine + phosphate | - |
? | |
| N-(5'-phospho-4'-pyridoxyl)ethanolamine + H2O | - |
Homo sapiens | 4'-pyridoxylethanolamine + phosphate | - |
? | |
| N-(5'-phospho-4'-pyridoxyl)glycine + H2O | - |
Homo sapiens | 4'-pyridoxylglycine + phosphate | - |
? | |
| N-(5'-phospho-4'-pyridoxyl)phenylalanine + H2O | much higher catalytic efficiency than with pyridoxine 5-phosphate | Homo sapiens | 4'-pyridoxylphenylalanine + phosphate | - |
? | |
| pyridoxal 5'-phosphate + H2O | highest catalytic efficiency with pyridoxal 5-phosphate and 4-pyridoxic acid 5-phosphate | Homo sapiens | pyridoxal + phosphate | - |
? | |
| pyridoxamine 5'-phosphate + H2O | low hydrolysis rate | Homo sapiens | pyridoxamine + phosphate | - |
? | |
| pyridoxine 5'-phosphate + H2O | - |
Homo sapiens | pyridoxine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at, inhibition studies | Homo sapiens |
| 37 | - |
assay at, kinetic analysis | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 60 min, no loss of activity | Homo sapiens |
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