Any feedback?
Literature summary for 3.1.3.74 extracted from
- Kinetic mechanism and divalent metal activation of human erythrocyte pyridoxal phosphatase (1995), Arch. Biochem. Biophys., 320, 345-352.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-ethyl-5-phenylisoxazolium-3'-sulfonate | inhibition is potentiated by MgCl2 | Homo sapiens |  |
| 5,5'-dithiobis(2-nitrobenzoate) | inhibition is potentiated by MgCl2 | Homo sapiens | |
| Ca2+ | competitive inhibition versus Mg2+, noncompetitive versus substrate | Homo sapiens | |
| Cd2+ | - |
Homo sapiens | |
| Cu2+ | - |
Homo sapiens | |
| diethyldicarbonate | inhibition is not potentiated by MgCl2 | Homo sapiens | |
| Fe2+ | - |
Homo sapiens | |
| iodoacetamide | weak | Homo sapiens | |
| iodoacetate | inhibition is potentiated by MgCl2 | Homo sapiens | |
| Mn2+ | inhibits above 0.05 mM, activates below 0.05 mM | Homo sapiens | |
| molybdate | competitive inhibition versus substrate, noncompetitive versus Mg2+ | Homo sapiens | |
| N-ethylmaleimide | inactivated by low concentrations, low concentrations of a substrate, pyridoxine phosphate, or phosphate protect from inactivation, inhibition is not potentiated by MgCl2 | Homo sapiens | |
| Pb2+ | - |
Homo sapiens | |
| Phenylglyoxal | inhibition is not potentiated by MgCl2 | Homo sapiens | |
| phosphate | competitive inhibition versus substrate, noncompetitive versus Mg2+ | Homo sapiens | |
| Zn2+ | inhibits at higher concentrations, activates somewhat at low concentrations | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic mechanism, kinetic properties | Homo sapiens | |
| 0.00154 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, 0.188 mM Mg2+ | Homo sapiens | |
| 0.00166 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, 0.06 mM Co2+ | Homo sapiens | |
| 0.0043 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, 0.39 mM Mg2+ | Homo sapiens | |
| 0.00812 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, 0.13 mM Co2+ | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | similar activation as by Mg2+ and Ni2+, kinetics | Homo sapiens | |
| Mg2+ | similar activation as by Co2+ and Ni2+, kinetics, probably the physiological activator | Homo sapiens | |
| Mn2+ | less activatory than Mg2+, Co2+ or Ni2+, inhibits above 0.05 mM, kinetics | Homo sapiens | |
| additional information | essential requirement for divalent cations | Homo sapiens | |
| Ni2+ | similar activation as by Mg2+ and Co2+, kinetics | Homo sapiens | |
| Zn2+ | activates somewhat at low concentrations, inhibits at higher concentrations | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | probably plays an important role in the regulation of vitamin B6 metabolism | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 10%, no effect on enzyme activity | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate | kinetic mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
pH 7.4, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | probably plays an important role in the regulation of vitamin B6 metabolism | Homo sapiens | ? | - |
? | |
| pyridoxal 5'-phosphate + H2O | kinetic mechanism, random binding of pyridoxal phosphate and Mg2+, formation of a dead-end complex of phosphate with the enzyme-Mg complex | Homo sapiens | pyridoxal + phosphate | - |
? | |
| pyridoxine 5'-phosphate + H2O | - |
Homo sapiens | pyridoxine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000 | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.00348 | - |
molybdate | - |
Homo sapiens | |
| 0.00638 | - |
Ca2+ | - |
Homo sapiens | |
| 0.22 | - |
phosphate | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
