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Literature summary for 3.1.3.74 extracted from

  • Gao, G.J.; Fonda, M.L.
    Evidence for a phosphoenzyme intermediate formed during catalysis by pyridoxal phosphatase from human erythrocytes (1994), Arch. Biochem. Biophys., 313, 166-172.
    View publication on PubMed
Search for more literature for 3.1.3.74

Inhibitors

Inhibitors Comment Organism Structure
2-ethyl-5-phenylisoxazolium-3'-sulfonate 0.25 mM, 5 min at 22°C, 60% loss of activity, inactivates in a concentration- and time-dependent manner, which follows pseudo-first-order kinetics, pyridoxal 5’-phosphate, pyridoxine 5’-phosphate or phosphate protects Homo sapiens
phosphate competitive inhibitor Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000 Homo sapiens
64000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxal 5'-phosphate + H2O Homo sapiens enzyme probably plays an important role in the hydrolysis of pyridoxal 5’-phosphate to pyridoxal in erythrocytes, may be important in the regulation of pyridoxal 5’-phosphate concentration pyridoxal + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate mechanism Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte outdated packed erythrocytes Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme has phosphotransferase activity and transfers 20-25% of the phosphoryl group from either substrate to ethanol Homo sapiens ?
-
 ?
pyridoxal 5'-phosphate + H2O enzyme probably plays an important role in the hydrolysis of pyridoxal 5’-phosphate to pyridoxal in erythrocytes, may be important in the regulation of pyridoxal 5’-phosphate concentration Homo sapiens pyridoxal + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O mechanism, a covalent phosphoenzyme intermediate is formed during catalysis, may be an acylphosphate intermediate Homo sapiens pyridoxal + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Homo sapiens