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Literature summary for 3.1.3.72 extracted from

  • Scott, J.J.; Loewus, F.A.
    A calcium-activated phytase from pollen of Lilium longiflorum (1986), Plant Physiol., 82, 333-335.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.72

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at concentrations equimolar to the substrate: 313% activation, but inhibitory at higher concentrations Lilium longiflorum
EDTA 0.5 mM: 35% inhibition, 5 mM: 94% inhibition Lilium longiflorum
Mg2+
-
 Lilium longiflorum
Mn2+
-
 Lilium longiflorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0072
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate in presence of Ca2+ Lilium longiflorum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at concentrations equimolar to the substrate: 313% activation, but inhibitory at higher concentrations Lilium longiflorum
additional information not activated by Mg2+, Mn2+, Cu2+ and Co2+ Lilium longiflorum
Sr2+ activates Lilium longiflorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 gel filtration Lilium longiflorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Lilium longiflorum enzyme activity may be regulated by the intracellular Ca2+ concentration myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lilium longiflorum
-
 cv. Nellie White
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, extraction with 0.1% Triton X-100 increases recovery by nearly 4fold Lilium longiflorum

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Lilium longiflorum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.066
-
-
 Lilium longiflorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O enzyme activity may be regulated by the intracellular Ca2+ concentration Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
 Lilium longiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 most active in Tris-maleate, rather than in Bicine buffer Lilium longiflorum