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Literature summary for 3.1.3.72 extracted from
- Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil (2007), J. Ind. Microbiol. Biotechnol., 34, 91-98.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | sucrose poorly supports phytase production. Maximal phytase yield is obtained with 2% (v/v) inoculum, phytase activity decreases at lower and higher inoculum concentrations | Sporolactobacillus laevolacticus | |
| NH4+ H2PO4- | can stimulate high-phytase production | Sporolactobacillus laevolacticus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | partially purified enzyme shows properties that are suitable for animal feed applications | Sporolactobacillus laevolacticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | moderately inhibits phytase activity | Sporolactobacillus laevolacticus | |
| EDTA | at 1 mM inhibits the enzyme activity up to 90% and completely at 5 mM concentration | Sporolactobacillus laevolacticus | |
| additional information | MnSO4, CaCl2, MgSO4, NaCl, KCl, BaCl2 and CoCl2 show insignificant effect on enzyme activity | Sporolactobacillus laevolacticus | |
| ZnSO4 | moderately inhibits phytase activity | Sporolactobacillus laevolacticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.526 | - |
Na-phytate | - |
Sporolactobacillus laevolacticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Ca2+ dependent | Sporolactobacillus laevolacticus | |
| Na+ | - |
Sporolactobacillus laevolacticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE | Sporolactobacillus laevolacticus |
| 46000 | - |
1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE | Sporolactobacillus laevolacticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sporolactobacillus laevolacticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified, 6.5fold, by gel filtration | Sporolactobacillus laevolacticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.94 | - |
crude extract | Sporolactobacillus laevolacticus |
| 12.69 | - |
partially purified phytase | Sporolactobacillus laevolacticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | 103% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
| AMP + H2O | 5% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
| ATP + H2O | 41% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
| Ca-phytate + H2O | 133% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
| additional information | no activity with riboflurin phosphate | Sporolactobacillus laevolacticus | ? | - |
? | |
| myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Sporolactobacillus laevolacticus | myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate | - |
? | |
| Na-phytate + H2O | 100% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | 5% relative activity | Sporolactobacillus laevolacticus | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | 32% relative activity | Sporolactobacillus laevolacticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE | Sporolactobacillus laevolacticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phytase | - |
Sporolactobacillus laevolacticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
partially purified phytase | Sporolactobacillus laevolacticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
ca. 80% activity at pH 8.0 for up to 3 h in the presence/absence of 5 mM CaCl2. In the presence/absence of 5 mM CaCl2, phytase is also stable at 80°C and shows ca. 100% of residual activity for 30 min at pH 8.0 and then declines sharply thereafter | Sporolactobacillus laevolacticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
partially purified phytase | Sporolactobacillus laevolacticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8.5 | is catalytically active under alkaline pH range 8-10, showing 90% of the optimal activity | Sporolactobacillus laevolacticus |
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