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Literature summary for 3.1.3.72 extracted from

  • Scott, J.J.
    Alkaline phytase activity in nonionic detergent extracts of legume seeds (1991), Plant Physiol., 95, 1298-1301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.72

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity Medicago sativa
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity Phaseolus vulgaris
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity Pisum sativum
additional information not inhibited by flouride Medicago sativa
additional information not inhibited by flouride Phaseolus vulgaris
additional information not inhibited by flouride Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity, 10 mM EGTA prevents the calcium effect Phaseolus vulgaris
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity, 10 mM EGTA prevents the calcium effect Pisum sativum
Ca2+ activates at a concentration equal to the substrate, but higher concentrations result in the precipitation of the substrate as calcium phytate and strongly inhibit enzyme activity, 10 mM EGTA prevents the calcium effect Medicago sativa

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-
Phaseolus vulgaris
-
 vars. Pinto, Navy, Red Kidney, Chili No. 494071, Netherlands No. 477020 and 486345, Black Beauty Turtle, Black Turtle G-0681 and T-39
-
Pisum sativum
-
 var. Early Alaska
-

Purification (Commentary)

Purification (Comment) Organism
in absence of Triton X-100, very little enzyme activity is extracted from seeds Phaseolus vulgaris
in absence of Triton X-100, very little enzyme activity is extracted from seeds Pisum sativum
in absence of Triton X-100, very little enzyme activity is extracted from seeds Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed dormant seeds Phaseolus vulgaris
-
seed dormant seeds Pisum sativum
-
seed dormant seeds Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Phaseolus vulgaris
additional information
-
-
 Pisum sativum
additional information
-
-
 Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O myo-inositol-1,2,3,4,5,6-hexakisphosphate is identical with phytic acid Phaseolus vulgaris myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O myo-inositol-1,2,3,4,5,6-hexakisphosphate is identical with phytic acid Pisum sativum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O myo-inositol-1,2,3,4,5,6-hexakisphosphate is identical with phytic acid Medicago sativa myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phytase
-
 Phaseolus vulgaris
alkaline phytase
-
 Pisum sativum
alkaline phytase
-
 Medicago sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Phaseolus vulgaris
37
-
 assay at Pisum sativum
37
-
 assay at Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Phaseolus vulgaris
8
-
-
 Pisum sativum
8
-
-
 Medicago sativa