Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-carboxyethylphosphonate | weak, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Ca2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Cd2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Cu+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Fe2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Fe3+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
Mg2+ | in presence of equimolar Mg2+, polyhistidine-tagged enzyme | Methanocaldococcus jannaschii | |
vanadate | - |
Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
(S)-phosphomalate | - |
Methanocaldococcus jannaschii | |
0.011 | - |
(R)-phosphomalate | - |
Methanocaldococcus jannaschii | |
0.034 | - |
Phosphoglycolate | - |
Methanocaldococcus jannaschii | |
0.063 | - |
rac-phosphosulfolactate | - |
Methanocaldococcus jannaschii | |
0.148 | - |
(S)-phospholactate | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
Cu2+ | weak activation | Methanocaldococcus jannaschii | |
Mg2+ | dependent on, at a concentration approximately equimolar with substrate | Methanocaldococcus jannaschii | |
Mn2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
Ni2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii | |
Zn2+ | can fully substitute for Mg2+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-3-sulfolactate + H2O | Methanocaldococcus jannaschii | the enzyme catalyzes the second step in biosynthesis of coenzyme M | 3-sulfolactate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48 | - |
rac-phosphosulfolactate | Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
-30°C, in presence of 50% v/v glycerol, 25% loss of activity after 3 weeks | Methanocaldococcus jannaschii |
4°C, 7% loss of activity after 3 weeks | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-phospholactate + H2O | - |
Methanocaldococcus jannaschii | lactate + phosphate | - |
? | |
(R)-phosphomalate + H2O | - |
Methanocaldococcus jannaschii | malate + phosphate | - |
? | |
(S)-2-phospholactate + H2O | no activity with (R)-phospholactate | Methanocaldococcus jannaschii | lactate + phosphate | - |
? | |
(S)-2-phosphomalate + H2O | - |
Methanocaldococcus jannaschii | malate + phosphate | - |
? | |
2-phospho-3-sulfolactate + H2O | the enzyme catalyzes the second step in biosynthesis of coenzyme M | Methanocaldococcus jannaschii | 3-sulfolactate + phosphate | - |
? | |
additional information | in presence of (S)-lactate the histidine-tagged enzyme can dephosphorylate phosphoglycolate and produce 2-phospholactate | Methanocaldococcus jannaschii | ? | - |
? | |
phosphoglycolate + H2O | - |
Methanocaldococcus jannaschii | glycolate + phosphate | - |
? | |
rac-2-phosphosulfolactate + H2O | - |
Methanocaldococcus jannaschii | sulfolactate + phosphate | - |
? | |
rac-2-phosphosulfolactate + H2O | - |
Methanosarcina thermophila | sulfolactate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | 65°C: about 50% of maximal activity, 85°C: about 70% of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | pH 4.5: about 80% of maximal activity, pH 7.0: about 55%of maximal activity, polyhistidine-tagged enzyme, hydrolysis of rac-phosphosulfolactate | Methanocaldococcus jannaschii |