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Literature summary for 3.1.3.69 extracted from

  • Roenneke, B.; Rosenfeldt, N.; Derya, S.M.; Novak, J.F.; Marin, K.; Kraemer, R.; Seibold, G.M.
    Production of the compatible solute alpha-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum (2018), Microb. Cell Fact., 17, 94 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis plasmid-bound expression of glucosylglycerol synthase and glucosylglycerol phosphatase from Synechocystis sp. PCC 6803 enables alpha-D-glucosylglycerol synthesis exclusively in osmotically stressed cells of Corynebacterium glutamicum. Upon deletion of both the glgA encoded glycogen synthase and the otsA encoded trehalose-6-phosphate synthase, the alpha-D-glucosylglycerol concentration in culture supernatants is increased from 0.5 mM in to 2.9 mM. Upon nitrogen limitation, transgenic Corynebacterium produces more than 10 mM alpha-D-glucosylglycerol (about 2 g/l) Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 Q55034
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