Literature summary for 3.1.3.66 extracted from

Gewinner, C.; Wang, Z.C.; Richardson, A.; Teruya-Feldstein, J.; Etemadmoghadam, D.; Bowtell, D.; Barretina, J.; Lin, W.M.; Rameh, L.; Salmena, L.; Pandolfi, P.P.; Cantley, L.C.
Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling (2009), Cancer Cell, 16, 115-125.

Search for more literature for 3.1.3.66

Application

Application	Comment	Organism
medicine	decreased expression of INPP4B correlates with poor outcome in both breast cancer and ovarian cancer patients, whereby the PI3K pathway is likely to play a major role in driving this subset of cancers. Loss of INPP4B expression may provide a marker for selecting patients who will respond to PI3K drugs	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
pLXSN-Flag-INPP4B expressed in 293T cells or in SUM149 cells subcutaneously injected into nude mice	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	rescue construct pLXSN/Flag-INPP4BR harbors two silent mutations at Ser687 to render it resistant to shRNA-INPP4B-1 knockdown. The catalytic-dead pLXSN/Flag-INPP4BR construct harbors a Cys842Ala mutation to abolish phosphatase activity as well as two silent mutations at Ser687 to avoid knockdown	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
mammary epithelial cell	-	Homo sapiens	-
MCF-10A cell	-	Homo sapiens	-
additional information	present in HMEC cells. Total level of INPP4B is approximately 2-3fold higher in SUM149 cells expressing Flag-INPP4B, compared with levels in control SUM149 cells	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-myo-phosphatidylinositol-3,4-bisphosphate + H2O	prefered substrate	Homo sapiens	D-myo-inositol 4-phosphate + phosphate	-	?
additional information	has no effect on phosphatidylinositol-1,3,4-triphosphate	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
inositol polyphosphate 4-phosphatase type II	-	Homo sapiens
INPP4B	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	knockdown of INPP4B expression by shRNA	down

General Information

General Information	Comment	Organism
malfunction	knocking down the expression of INPP4B in human epithelial cells, like knockdown of PTEN, results in enhanced Akt activation and anchorage-independent growth and enhanced overall motility. INPP4B knockdown results in increased migratory and invasive behavior of mammary epithelial cells. Dual knockdown of INPP4B and PTEN results in cellular senescence and causes a more prolonged phosphorylation of AKT at Thr308 than that caused by knocking down either protein alone. INPP4B knockdown in MCF-10A mammary epithelial cells results in distorted acini architecture in three-dimensional culture	Homo sapiens
physiological function	overexpression of INPP4B in SUM149 cells in a xenograft mouse model results in reduced tumor growth. Loss of heterozygosity at the INPP4B locus in a majority of basal-like breast cancers, as well as in a significant fraction of ovarian cancers, which correlates with lower overall patient survival, suggesting that INPP4B is a tumor suppressor	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)