Literature summary for 3.1.3.64 extracted from

Franklin, N.E.; Bonham, C.A.; Xhabija, B.; Vacratsis, P.O.
Differential phosphorylation of the phosphoinositide 3-phosphatase MTMR2 regulates its association with early endosomal subtypes (2013), J. Cell Sci., 126, 1333-1344.

Search for more literature for 3.1.3.64

Protein Variants

Protein Variants	Comment	Organism
S58A	comparison with double mutant S58A/S631A shows that dephosphorylation of Ser58 is sufficient for targeting MTMR2 to endosomal structures	Homo sapiens
S58A/C417S/S631A	catalytically inactive variant. Loss of catalytic activity (S58A/C417S/S631A) of dephosphorylated MTMR2 results in partial colocalization with PI(3)P endosomes. In contrast to double mutant S58A/S631A triple mutant S58A/C417S/S631A containing an additional mutated catalytic residue do not show enlarged viscles being positive for both MTMR2 and APPL1. This underlines that that the phosphatase activity of MTMR2 contributes to the observed enlargement of MTMR2/APPL1 positive vesicles	Homo sapiens
S58A/S631A	dephosphorylation at Ser58 and Ser631 in double mutant S58A/S631A prevents localization to PI(3)P-rich endosomes. MTMR2 localization shift upon administration of MAPK inhibitors is recapitulated in double mutant S58A/S631A. Expression of this double mutant leads to a more sustained and pronounced increase in ERK1/2 activation compared with mutant S58A. Further analysis of single mutant S58A and double mutant S58A/S631A demonstrate that it is the phosphorylation status of Ser58 that regulates general endosomal binding and that the phosphorylation status of Ser631 mediates the endosomal shuttling between Rab5 and APPL1 subtypes	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome	treatment with multiple MAPK inhibitors results in a MTMR2 localization shift from Rab5-positive endosomes to the more proximal APPL1-positive endosomes	Homo sapiens	5768	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	ERK1/2 is the kinase responsible for phosphorylating MTMR2 at position Ser58, which suggests that the endosomal targeting of MTMR2 is regulated through an ERK1/2 negative feedback mechanism	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
MTMR2	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)