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Literature summary for 3.1.3.64 extracted from

  • Toulabi, L.; Wu, X.; Cheng, Y.; Mao, Y.
    Identification and structural characterization of a Legionella phosphoinositide phosphatase (2013), J. Biol. Chem., 288, 24518-24527.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Legionella pneumophila
expressed in Escherichia coli as a His-tagged fusion protein Legionella longbeachae

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of SidP orthologue from Legionella longbeachae reveals that this unique PI-3-phosphatase is composed of three distinct domains: a large catalytic domain, an appendage domain that is inserted into the N-terminal portion of the catalytic domain, and a C-terminalalpha-helical domain Legionella pneumophila

Protein Variants

Protein Variants Comment Organism
C554S mutant does not show any activity Legionella pneumophila
D559N mutant does not show any activity Legionella pneumophila
R560K mutant does not show any activity Legionella pneumophila

Organism

Organism UniProt Comment Textmining
Legionella longbeachae D3HMN4
-
-
Legionella longbeachae NSW150 D3HMN4
-
-
Legionella pneumophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Legionella pneumophila
-
 Legionella longbeachae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Legionella pneumophila 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O
-
 Legionella pneumophila 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O enzyme shows specific enzymatic activity against PI(3)P but no detectable activity against PI(3,5)P2 Legionella longbeachae 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O enzyme shows specific enzymatic activity against PI(3)P but no detectable activity against PI(3,5)P2 Legionella longbeachae NSW150 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
SidP
-
 Legionella pneumophila
SidP
-
 Legionella longbeachae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Legionella pneumophila
37
-
 assay at Legionella longbeachae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Legionella pneumophila
8
-
 assay at Legionella longbeachae

General Information

General Information Comment Organism
malfunction activity of SidP rescues the growth phenotype of a yeast strain defective in PI(3)P phosphatase activity Legionella pneumophila