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Literature summary for 3.1.3.63 extracted from
- Purification and properties of a phosphatase in French bean (Phaseolus vulgaris L.) leaves that hydrolyses 2-carboxy-D-arabinitol 1-phosphate (1992), Biochem. J., 287, 821-825.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-bisphosphate | activation | Phaseolus vulgaris |  |
| D-fructose 1,6-bisphosphate | 2 mM | Phaseolus vulgaris | |
| additional information | no activation by dithiothreitol | Phaseolus vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
2-carboxyarabinitol 1-phosphate | 25°C, pH 7.5 | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Phaseolus vulgaris | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
| CaCl2 | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
| KCl | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
| MgCl2 | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
| NaCl | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
| NaHCO3 | activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2 | Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-carboxy-D-arabinitol 1-phosphate + H2O | Phaseolus vulgaris | - |
2-carboxy-D-arabinitol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
French bean, var. Tendergreen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.71 | - |
- |
Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, several months | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-carboxy-D-arabinitol 1,5-bisphosphate + H2O | - |
Phaseolus vulgaris | 2-carboxy-D-arabinitol 5-phosphate + phosphate | - |
? | |
| 2-carboxy-D-arabinitol 1-phosphate + H2O | - |
Phaseolus vulgaris | 2-carboxy-D-arabinitol + phosphate | - |
? | |
| 2-carboxy-D-ribitol 1,5-bisphosphate + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| D-3-phosphoglycerate + H2O | poor substrate | Phaseolus vulgaris | ? | - |
? | |
| diphosphate + H2O | poor substrate | Phaseolus vulgaris | 2 phosphate | - |
? | |
| fructose 1,6-bisphosphate + H2O | poor substrate | Phaseolus vulgaris | ? | - |
? | |
| additional information | no substrates are D-glucose 1-phosphate, D-glucose 6-phosphate | Phaseolus vulgaris | ? | - |
? | |
| additional information | no substrates are phosphoglycolic acid, D-fructose 6-phosphate, D-sedoheptulose 1,7-bisphosphate, NADP+, ATP, 5'-AMP | Phaseolus vulgaris | ? | - |
? | |
| additional information | no substrates are D-6-phosphogluconate | Phaseolus vulgaris | ? | - |
? | |
| ribulose 1,5-bisphosphate + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | about 75% of maximal activity at pH 6 and about half-maximal activity at pH 8.5 | Phaseolus vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | activation | Phaseolus vulgaris | |
| NADPH | 2 mM | Phaseolus vulgaris | |
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