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Literature summary for 3.1.3.62 extracted from
- Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) multiple inositol polyphosphate phosphatases (MINPPs) are phytases expressed during grain filling and germination (2007), Plant Biotechnol. J., 5, 325-338.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Hordeum vulgare |  |
| Cu2+ | - |
Triticum aestivum | |
| Zn2+ | - |
Hordeum vulgare | |
| Zn2+ | - |
Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.246 | - |
Inositol hexakisphosphate | - |
Triticum aestivum | |
| 0.334 | - |
Inositol hexakisphosphate | - |
Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | all cDNAs contain an N-terminal signal peptide encoding sequence, and a KDEL-like sequence, KTEL, is present at the C-terminal, indicating that the enzyme is targeted to and retained within the endoplasmic reticulum | Hordeum vulgare | 5783 | - |
| endoplasmic reticulum | all cDNAs contain an N-terminal signal peptide encoding sequence, and a KDEL-like sequence, KTEL, is present at the C-terminal, indicating that the enzyme is targeted to and retained within the endoplasmic reticulum | Triticum aestivum | 5783 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | A0FHA7 | - |
- |
| Hordeum vulgare | A0FHA8 | - |
- |
| Hordeum vulgare | A0FHA9 | - |
- |
| Triticum aestivum | A0FHB0 | - |
- |
| Triticum aestivum | A0FHB1 | - |
- |
| Triticum aestivum | A0FHB2 | - |
- |
| Triticum aestivum | A0FHB3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cDNAs show significantly different temporal and tissue-specific expression patterns during seed development and germination. With the exception of TaPhyIIb, the cDNAs are present during late seed development and germination | Hordeum vulgare | - |
| additional information | cDNAs show significantly different temporal and tissue-specific expression patterns during seed development and germination. With the exception of TaPhyIIb, the cDNAs are present during late seed development and germination | Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
expression of HvPhyIIb in Escherichia coli reveals that the MINPPs possesses a significant phytase activity with narrow substrate specificity for phytate | Hordeum vulgare |
| additional information | - |
expression of TaPhyIIa2 in Escherichia coli reveals that the MINPPs possesses a significant phytase activity with narrow substrate specificity for phytate | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inositol hexakisphosphate + H2O | - |
Triticum aestivum | inositol pentakisphosphate + phosphate | - |
? | |
| inositol hexakisphosphate + H2O | - |
Hordeum vulgare | inositol pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HvPhyIIa1 | - |
Hordeum vulgare |
| HvPhyIIa2 | - |
Hordeum vulgare |
| HvPhyIIb | - |
Hordeum vulgare |
| Minpp | - |
Hordeum vulgare |
| Minpp | - |
Triticum aestivum |
| multiple inositol polyphosphate phosphatase | - |
Hordeum vulgare |
| multiple inositol polyphosphate phosphatase | - |
Triticum aestivum |
| TaPhyIIa1 | - |
Triticum aestivum |
| TaPhyIIa2 | - |
Triticum aestivum |
| TaPhyIIb | - |
Triticum aestivum |
| TaPhyIIc | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Triticum aestivum |
| 65 | - |
- |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Triticum aestivum |
| 4.5 | - |
- |
Hordeum vulgare |
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