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Literature summary for 3.1.3.62 extracted from
- Properties of a soluble inositol 1,3,4,5-tetrakisphosphate 3-phosphatase from porcine brain (1990), Biochem. J., 270, 715-719.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | weak | Sus scrofa |  |
| CTP | more effective inhibitor than GTP | Sus scrofa | |
| GTP | marked inhibition | Sus scrofa | |
| Inositol 1,3,4,5,6-pentakisphosphate | - |
Sus scrofa | |
| inositol 1,3,4-trisphosphate | mixed-type inhibition | Sus scrofa | |
| Inositol 1,4,5-trisphosphate | product inhibition kinetics | Sus scrofa | |
| inositol 3,4,5,6-tetrakisphosphate | commercially available with 12% inositol 2,4,5,6-tetrakisphosphate, from turkey erythrocytes, marked inhibition, linear mixed-type inhibition | Sus scrofa | |
| additional information | not inhibited by Mg2+, Ca2+, Li+, non-hydrolysable adenine, guanine nucleotides or deoxynucleotides | Sus scrofa | |
| UTP | more effective inhibitor than GTP | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
D-myo-Inositol 1,3,4,5-tetrakisphosphate | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Sus scrofa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activation | Sus scrofa | |
| additional information | not activated by Ca2+ up to 1 mM or Mg2+ up to 30 mM | Sus scrofa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | Sus scrofa | enzyme may be regulated by the intracellular concentrations of inositol tris- and tetrakisphosphates | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, several months, stable | Sus scrofa |
| 4°C, within weeks, loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Sus scrofa | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
| D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | enzyme may be regulated by the intracellular concentrations of inositol tris- and tetrakisphosphates | Sus scrofa | D-myo-inositol 1,4,5-trisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | may be identical with EC 3.1.3.65 | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
inositol 3,4,5,6-tetrakisphosphate | pH 7.4, 37°C | Sus scrofa | |
| 0.00175 | - |
inositol 1,3,4-trisphosphate | pH 7.4, 37°C | Sus scrofa | |
| 0.002 | - |
Inositol 1,4,5-trisphosphate | pH 7.4, 37°C | Sus scrofa | |
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