BRENDA - Enzyme Database
show all sequences of 3.1.3.60

Partial purification and characterization of phosphoenolpyruvate phosphatase from developing seeds of Brassica campestris

Singh, D.K.; Singh, R.; Proc. Indian Acad. Sci. Sect. B 66, 53-63 (1996)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-phosphoglycerate
-
Brassica rapa subsp. oleifera
Cu2+
-
Brassica rapa subsp. oleifera
Fe2+
-
Brassica rapa subsp. oleifera
molybdate
-
Brassica rapa subsp. oleifera
NaF
-
Brassica rapa subsp. oleifera
Zn2+
-
Brassica rapa subsp. oleifera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
phosphoenolpyruvate
-
Brassica rapa subsp. oleifera
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Brassica rapa subsp. oleifera
Co2+
-
Brassica rapa subsp. oleifera
Mg2+
-
Brassica rapa subsp. oleifera
Mn2+
-
Brassica rapa subsp. oleifera
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Brassica rapa subsp. oleifera
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Brassica rapa subsp. oleifera
-
pyruvate + phosphate
-
Brassica rapa subsp. oleifera
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica rapa subsp. oleifera
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Brassica rapa subsp. oleifera
Storage Stability
Storage Stability
Organism
-15-4°C, 50 days, no loss of activity
Brassica rapa subsp. oleifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphoenolpyruvate + H2O
-
646427
Brassica rapa subsp. oleifera
pyruvate + phosphate
-
646427
Brassica rapa subsp. oleifera
?
phosphoenolpyruvate + H2O
relatively unspecific
646427
Brassica rapa subsp. oleifera
pyruvate + phosphate
-
646427
Brassica rapa subsp. oleifera
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-phosphoglycerate
-
Brassica rapa subsp. oleifera
Cu2+
-
Brassica rapa subsp. oleifera
Fe2+
-
Brassica rapa subsp. oleifera
molybdate
-
Brassica rapa subsp. oleifera
NaF
-
Brassica rapa subsp. oleifera
Zn2+
-
Brassica rapa subsp. oleifera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
phosphoenolpyruvate
-
Brassica rapa subsp. oleifera
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Brassica rapa subsp. oleifera
Co2+
-
Brassica rapa subsp. oleifera
Mg2+
-
Brassica rapa subsp. oleifera
Mn2+
-
Brassica rapa subsp. oleifera
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
gel filtration
Brassica rapa subsp. oleifera
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Brassica rapa subsp. oleifera
-
pyruvate + phosphate
-
Brassica rapa subsp. oleifera
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Brassica rapa subsp. oleifera
Storage Stability (protein specific)
Storage Stability
Organism
-15-4°C, 50 days, no loss of activity
Brassica rapa subsp. oleifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphoenolpyruvate + H2O
-
646427
Brassica rapa subsp. oleifera
pyruvate + phosphate
-
646427
Brassica rapa subsp. oleifera
?
phosphoenolpyruvate + H2O
relatively unspecific
646427
Brassica rapa subsp. oleifera
pyruvate + phosphate
-
646427
Brassica rapa subsp. oleifera
?
Other publictions for EC 3.1.3.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731415
Yang
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Magnesium deficiency-induced c ...
Citrus sinensis
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481-486
2013
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3
1
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1
1
-
-
-
680386
Smith
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Iron assimilation and carbon m ...
Vitis labrusca x Vitis vinifera
J. Am. Soc. Hort. Sci.
132
473-483
2007
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1
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1
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663038
Suzuki
-
Salt stress and glycolytic reg ...
Bruguiera sexangula
Physiol. Plant.
123
246-253
2005
1
-
-
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-
1
-
1
1
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1
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1
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1
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1
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2
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1
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2
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666551
Obiadalla-Ali
Developmental analysis of carb ...
Solanum lycopersicum
Physiol. Plant.
120
196-204
2004
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1
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2
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1
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666554
Dong
-
Organic acid exudation induced ...
Glycine max
Physiol. Plant.
122
190-199
2004
1
-
-
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-
1
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1
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1
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1
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1
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646426
Shinano
-
Characteristics of phosphoenol ...
Allium cepa
Plant Sci.
161
861-869
2001
-
-
-
-
-
-
2
6
-
1
2
1
-
1
-
-
1
-
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
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-
-
-
-
-
-
-
-
2
-
6
-
1
2
1
-
-
-
1
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
646427
Singh
-
Partial purification and chara ...
Brassica rapa subsp. oleifera
Proc. Indian Acad. Sci. Sect. B
66
53-63
1996
-
-
-
-
-
-
6
1
-
4
1
1
-
1
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
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-
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6
-
1
-
4
1
1
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1
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1
2
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-
-
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-
-
-
134800
Duff
Purification, characterization ...
Brassica nigra
Arch. Biochem. Biophys.
286
226-232
1991
-
-
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-
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1
-
1
1
-
2
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1
1
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1
1
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10
1
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1
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1
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1
1
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1
1
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1
1
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10
1
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-
1
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646424
Podesta
Association of phosphoenolpyru ...
Vigna radiata
Plant Physiol.
97
1329-1333
1991
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1
1
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6
-
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1
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1
1
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1
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1
1
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1
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1
1
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1
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-
646423
Malhotra
Isolation and characterization ...
Vigna radiata
Plant Physiol.
93
194-200
1990
-
-
-
-
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-
9
1
-
1
2
1
-
2
-
-
1
-
-
1
1
1
8
1
-
1
1
-
1
1
-
-
7
-
-
-
-
-
-
-
-
-
-
9
7
1
-
1
2
1
-
-
-
1
-
1
1
1
8
1
-
1
1
-
1
1
-
-
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-
-
-
-
-
646421
Duff
Purification and characterizat ...
Brassica nigra
Plant Physiol.
90
734-741
1989
-
-
-
-
-
-
14
10
-
4
2
1
-
5
-
-
1
-
-
3
2
1
18
1
-
-
3
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
14
4
10
-
4
2
1
-
-
-
1
-
3
2
1
18
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
646422
Malhotra
-
Chemical inactivation and acti ...
Vigna radiata
Plant Sci.
65
161-170
1989
-
-
-
-
-
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5
-
-
-
1
1
-
1
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1
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1
1
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-
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-
1
1
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-
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5
-
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1
1
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1
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1
1
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-
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1
1
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