Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.60 extracted from

  • Shinano, T.; Yonetani, R.; Ushihara, N.; Adachi, H.; Wasaki, J.; Matsui, H.; Osaki, M.
    Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa (2001), Plant Sci., 161, 861-869.
No PubMed abstract available
Search for more literature for 3.1.3.60

Inhibitors

Inhibitors Comment Organism Structure
F-
-
 Allium cepa
phosphate
-
 Allium cepa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 phosphoenolpyruvate 25°C, pH 6.6 Allium cepa
1.36
-
 phytic acid 25°C, pH 6.6 Allium cepa
1.47
-
 D-glucose 6-phosphate 25°C, pH 6.6 Allium cepa
1.51
-
 ATP 25°C, pH 6.6 Allium cepa
1.6
-
 4-nitrophenyl phosphate 25°C, pH 6.6 Allium cepa
2.69
-
 ADP 25°C, pH 6.6 Allium cepa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.7 fold activation at 5 mM Allium cepa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 SDS-PAGE, degradation product with 42000 Da Allium cepa
240000
-
 gel filtration Allium cepa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + H2O Allium cepa
-
 pyruvate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Allium cepa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
 purified enzyme, 25°C, pH 6.6 Allium cepa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Allium cepa 4-nitrophenol + phosphate
-
 ?
ADP + H2O
-
 Allium cepa ?
-
 ?
ATP + H2O
-
 Allium cepa ?
-
 ?
D-glucose 6-phosphate + H2O
-
 Allium cepa D-glucose + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Allium cepa pyruvate + phosphate
-
 ?
phytic acid + H2O
-
 Allium cepa ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 52000, gel filtration, SDS-PAGE Allium cepa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to 50°C Allium cepa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Allium cepa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 7.8 stable Allium cepa