BRENDA - Enzyme Database
show all sequences of 3.1.3.60

Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa

Shinano, T.; Yonetani, R.; Ushihara, N.; Adachi, H.; Wasaki, J.; Matsui, H.; Osaki, M.; Plant Sci. 161, 861-869 (2001)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
F-
-
Allium cepa
phosphate
-
Allium cepa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
phosphoenolpyruvate
25°C, pH 6.6
Allium cepa
1.36
-
phytic acid
25°C, pH 6.6
Allium cepa
1.47
-
D-glucose 6-phosphate
25°C, pH 6.6
Allium cepa
1.51
-
ATP
25°C, pH 6.6
Allium cepa
1.6
-
4-nitrophenyl phosphate
25°C, pH 6.6
Allium cepa
2.69
-
ADP
25°C, pH 6.6
Allium cepa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
1.7 fold activation at 5 mM
Allium cepa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE, degradation product with 42000 Da
Allium cepa
240000
-
gel filtration
Allium cepa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Allium cepa
-
pyruvate + phosphate
-
Allium cepa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Allium cepa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Allium cepa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
53
-
purified enzyme, 25°C, pH 6.6
Allium cepa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
646426
Allium cepa
4-nitrophenol + phosphate
-
-
-
?
ADP + H2O
-
646426
Allium cepa
?
-
-
-
?
ATP + H2O
-
646426
Allium cepa
?
-
-
-
-
D-glucose 6-phosphate + H2O
-
646426
Allium cepa
D-glucose + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
-
646426
Allium cepa
pyruvate + phosphate
-
646426
Allium cepa
?
phytic acid + H2O
-
646426
Allium cepa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 52000, gel filtration, SDS-PAGE
Allium cepa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to 50°C
Allium cepa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Allium cepa
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.7
7.8
stable
Allium cepa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
F-
-
Allium cepa
phosphate
-
Allium cepa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
phosphoenolpyruvate
25°C, pH 6.6
Allium cepa
1.36
-
phytic acid
25°C, pH 6.6
Allium cepa
1.47
-
D-glucose 6-phosphate
25°C, pH 6.6
Allium cepa
1.51
-
ATP
25°C, pH 6.6
Allium cepa
1.6
-
4-nitrophenyl phosphate
25°C, pH 6.6
Allium cepa
2.69
-
ADP
25°C, pH 6.6
Allium cepa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
1.7 fold activation at 5 mM
Allium cepa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
SDS-PAGE, degradation product with 42000 Da
Allium cepa
240000
-
gel filtration
Allium cepa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Allium cepa
-
pyruvate + phosphate
-
Allium cepa
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Allium cepa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
53
-
purified enzyme, 25°C, pH 6.6
Allium cepa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
646426
Allium cepa
4-nitrophenol + phosphate
-
-
-
?
ADP + H2O
-
646426
Allium cepa
?
-
-
-
?
ATP + H2O
-
646426
Allium cepa
?
-
-
-
-
D-glucose 6-phosphate + H2O
-
646426
Allium cepa
D-glucose + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
-
646426
Allium cepa
pyruvate + phosphate
-
646426
Allium cepa
?
phytic acid + H2O
-
646426
Allium cepa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 52000, gel filtration, SDS-PAGE
Allium cepa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to 50°C
Allium cepa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Allium cepa
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.7
7.8
stable
Allium cepa
Other publictions for EC 3.1.3.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731415
Yang
-
Magnesium deficiency-induced c ...
Citrus sinensis
Biol. Plant.
57
481-486
2013
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1
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3
1
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1
1
-
-
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680386
Smith
-
Iron assimilation and carbon m ...
Vitis labrusca x Vitis vinifera
J. Am. Soc. Hort. Sci.
132
473-483
2007
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1
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3
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1
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663038
Suzuki
-
Salt stress and glycolytic reg ...
Bruguiera sexangula
Physiol. Plant.
123
246-253
2005
1
-
-
-
-
-
1
-
1
1
-
1
-
1
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1
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2
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1
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2
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1
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1
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1
1
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1
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1
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2
-
1
-
-
-
2
-
-
-
-
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-
-
-
-
666551
Obiadalla-Ali
Developmental analysis of carb ...
Solanum lycopersicum
Physiol. Plant.
120
196-204
2004
-
-
-
-
-
-
-
-
-
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-
1
-
2
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2
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1
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1
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2
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
666554
Dong
-
Organic acid exudation induced ...
Glycine max
Physiol. Plant.
122
190-199
2004
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
-
1
-
-
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-
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
646426
Shinano
-
Characteristics of phosphoenol ...
Allium cepa
Plant Sci.
161
861-869
2001
-
-
-
-
-
-
2
6
-
1
2
1
-
1
-
-
1
-
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
1
2
1
-
-
-
1
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
646427
Singh
-
Partial purification and chara ...
Brassica rapa subsp. oleifera
Proc. Indian Acad. Sci. Sect. B
66
53-63
1996
-
-
-
-
-
-
6
1
-
4
1
1
-
1
-
-
1
-
-
-
-
1
2
-
-
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-
-
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6
-
1
-
4
1
1
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1
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-
1
2
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
134800
Duff
Purification, characterization ...
Brassica nigra
Arch. Biochem. Biophys.
286
226-232
1991
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
1
1
-
-
1
1
-
10
1
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
1
-
1
1
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
646424
Podesta
Association of phosphoenolpyru ...
Vigna radiata
Plant Physiol.
97
1329-1333
1991
-
-
-
-
-
-
-
-
-
-
1
1
-
6
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646423
Malhotra
Isolation and characterization ...
Vigna radiata
Plant Physiol.
93
194-200
1990
-
-
-
-
-
-
9
1
-
1
2
1
-
2
-
-
1
-
-
1
1
1
8
1
-
1
1
-
1
1
-
-
7
-
-
-
-
-
-
-
-
-
-
9
7
1
-
1
2
1
-
-
-
1
-
1
1
1
8
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
646421
Duff
Purification and characterizat ...
Brassica nigra
Plant Physiol.
90
734-741
1989
-
-
-
-
-
-
14
10
-
4
2
1
-
5
-
-
1
-
-
3
2
1
18
1
-
-
3
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
14
4
10
-
4
2
1
-
-
-
1
-
3
2
1
18
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
646422
Malhotra
-
Chemical inactivation and acti ...
Vigna radiata
Plant Sci.
65
161-170
1989
-
-
-
-
-
-
5
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-