BRENDA - Enzyme Database
show all sequences of 3.1.3.60

Isolation and characterization of phosphoenolpyruvate phosphatase from germinating mung beans (Vigna radiata)

Malhotra, O.P.; Kayastha, A.M.; Plant Physiol. 93, 194-200 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-phosphoglycerate
competitive
Vigna radiata
ATP
competitive
Vigna radiata
citrate
competitive
Vigna radiata
Cu2+
competitive towards Mg2+
Vigna radiata
Hg2+
competitive towards Mg2+
Vigna radiata
Ni2+
competitive towards Mg2+
Vigna radiata
oxalate
competitive
Vigna radiata
succinate
competitive
Vigna radiata
Zn2+
competitive towards Mg2+
Vigna radiata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
phosphoenolpyruvate
30°C, pH 8.5
Vigna radiata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Vigna radiata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Vigna radiata
240000
-
gel filtration
Vigna radiata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Vigna radiata
-
pyruvate + phosphate
-
Vigna radiata
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vigna radiata
-
mung bean
-
Purification (Commentary)
Commentary
Organism
-
Vigna radiata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
geminating bean; highest activity at 36 h of germination
Vigna radiata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.05
-
Vmax, purified enzyme, 30°C, pH 8.5
Vigna radiata
Storage Stability
Storage Stability
Organism
-10°C, purified enzyme, 50 days, 50% loss of activity
Vigna radiata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
ATP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
diphosphate + H2O
poor substrate
646423
Vigna radiata
2 phosphate
-
-
-
?
GDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
IDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
phosphoenolpyruvate + H2O
-
646423
Vigna radiata
pyruvate + phosphate
-
646423
Vigna radiata
?
phosphoenolpyruvate + H2O
highly specific
646423
Vigna radiata
pyruvate + phosphate
-
646423
Vigna radiata
?
UDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 60000, gel filtration, SDS-PAGE
Vigna radiata
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
-
Vigna radiata
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
half-life: 6.8 min, pH 6.5, 2.4 min, pH 7.5, 1.8 min, pH 8.6
Vigna radiata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Vigna radiata
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
-
Vigna radiata
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.023
-
Zn2+
competitive towards Mg2+, 30°C, pH 8.5
Vigna radiata
0.22
-
Cu2+
competitive towards Mg2+, 30°C, pH 8.5
Vigna radiata
3
-
oxalate
competitive, 30°C, pH 8.5
Vigna radiata
3.3
-
3-phosphoglycerate
competitive, 30°C, pH 8.5
Vigna radiata
4.5
-
citrate
competitive, 30°C, pH 8.5
Vigna radiata
5
-
ATP
competitive, 30°C, pH 8.5
Vigna radiata
6.3
-
succinate
competitive, 30°C, pH 8.5
Vigna radiata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-phosphoglycerate
competitive
Vigna radiata
ATP
competitive
Vigna radiata
citrate
competitive
Vigna radiata
Cu2+
competitive towards Mg2+
Vigna radiata
Hg2+
competitive towards Mg2+
Vigna radiata
Ni2+
competitive towards Mg2+
Vigna radiata
oxalate
competitive
Vigna radiata
succinate
competitive
Vigna radiata
Zn2+
competitive towards Mg2+
Vigna radiata
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.023
-
Zn2+
competitive towards Mg2+, 30°C, pH 8.5
Vigna radiata
0.22
-
Cu2+
competitive towards Mg2+, 30°C, pH 8.5
Vigna radiata
3
-
oxalate
competitive, 30°C, pH 8.5
Vigna radiata
3.3
-
3-phosphoglycerate
competitive, 30°C, pH 8.5
Vigna radiata
4.5
-
citrate
competitive, 30°C, pH 8.5
Vigna radiata
5
-
ATP
competitive, 30°C, pH 8.5
Vigna radiata
6.3
-
succinate
competitive, 30°C, pH 8.5
Vigna radiata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
phosphoenolpyruvate
30°C, pH 8.5
Vigna radiata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Vigna radiata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Vigna radiata
240000
-
gel filtration
Vigna radiata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phosphoenolpyruvate + H2O
Vigna radiata
-
pyruvate + phosphate
-
Vigna radiata
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Vigna radiata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
geminating bean; highest activity at 36 h of germination
Vigna radiata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.05
-
Vmax, purified enzyme, 30°C, pH 8.5
Vigna radiata
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, purified enzyme, 50 days, 50% loss of activity
Vigna radiata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
ATP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
diphosphate + H2O
poor substrate
646423
Vigna radiata
2 phosphate
-
-
-
?
GDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
IDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
phosphoenolpyruvate + H2O
-
646423
Vigna radiata
pyruvate + phosphate
-
646423
Vigna radiata
?
phosphoenolpyruvate + H2O
highly specific
646423
Vigna radiata
pyruvate + phosphate
-
646423
Vigna radiata
?
UDP + H2O
poor substrate
646423
Vigna radiata
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 60000, gel filtration, SDS-PAGE
Vigna radiata
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
-
Vigna radiata
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
half-life: 6.8 min, pH 6.5, 2.4 min, pH 7.5, 1.8 min, pH 8.6
Vigna radiata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Vigna radiata
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
-
Vigna radiata
Other publictions for EC 3.1.3.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731415
Yang
-
Magnesium deficiency-induced c ...
Citrus sinensis
Biol. Plant.
57
481-486
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
680386
Smith
-
Iron assimilation and carbon m ...
Vitis labrusca x Vitis vinifera
J. Am. Soc. Hort. Sci.
132
473-483
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
3
-
-
1
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-
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-
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3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663038
Suzuki
-
Salt stress and glycolytic reg ...
Bruguiera sexangula
Physiol. Plant.
123
246-253
2005
1
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
666551
Obiadalla-Ali
Developmental analysis of carb ...
Solanum lycopersicum
Physiol. Plant.
120
196-204
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666554
Dong
-
Organic acid exudation induced ...
Glycine max
Physiol. Plant.
122
190-199
2004
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646426
Shinano
-
Characteristics of phosphoenol ...
Allium cepa
Plant Sci.
161
861-869
2001
-
-
-
-
-
-
2
6
-
1
2
1
-
1
-
-
1
-
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
1
2
1
-
-
-
1
-
-
1
-
6
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
646427
Singh
-
Partial purification and chara ...
Brassica rapa subsp. oleifera
Proc. Indian Acad. Sci. Sect. B
66
53-63
1996
-
-
-
-
-
-
6
1
-
4
1
1
-
1
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
4
1
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134800
Duff
Purification, characterization ...
Brassica nigra
Arch. Biochem. Biophys.
286
226-232
1991
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
1
1
-
-
1
1
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
1
-
1
1
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
646424
Podesta
Association of phosphoenolpyru ...
Vigna radiata
Plant Physiol.
97
1329-1333
1991
-
-
-
-
-
-
-
-
-
-
1
1
-
6
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646423
Malhotra
Isolation and characterization ...
Vigna radiata
Plant Physiol.
93
194-200
1990
-
-
-
-
-
-
9
1
-
1
2
1
-
2
-
-
1
-
-
1
1
1
8
1
-
1
1
-
1
1
-
-
7
-
-
-
-
-
-
-
-
-
-
9
7
1
-
1
2
1
-
-
-
1
-
1
1
1
8
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
646421
Duff
Purification and characterizat ...
Brassica nigra
Plant Physiol.
90
734-741
1989
-
-
-
-
-
-
14
10
-
4
2
1
-
5
-
-
1
-
-
3
2
1
18
1
-
-
3
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
14
4
10
-
4
2
1
-
-
-
1
-
3
2
1
18
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
646422
Malhotra
-
Chemical inactivation and acti ...
Vigna radiata
Plant Sci.
65
161-170
1989
-
-
-
-
-
-
5
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-