Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | competitive; complete inhibition at 5 mM | Brassica nigra | |
aspartate | competitive | Brassica nigra | |
CuSO4 | complete inhibition at 4 mM | Brassica nigra | |
D-fructose 1,6-bisphosphate | complete inhibition at 5 mM, competitive | Brassica nigra | |
FeCl3 | complete inhibition at 4 mM | Brassica nigra | |
glutamate | competitive | Brassica nigra | |
glyceraldhyde-3-phosphate | competitive | Brassica nigra | |
Lead acetate | 70% inhibition at 8 mM | Brassica nigra | |
MgADP | 41% inhibition at 0.5 mM, competitive | Brassica nigra | |
molybdate | complete inhibition at 0.1 mM, competitive | Brassica nigra | |
additional information | several more substances found to be inhibitory, no inhibition with N-ethylmaleimide | Brassica nigra | |
NaF | uncompetitive | Brassica nigra | |
phosphate | complete inhibition at 0.5 mM, competitive | Brassica nigra | |
ZnCl2 | complete inhibition at 4 mM | Brassica nigra |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
phosphoenolpyruvate | 25°C, pH 5.6 | Brassica nigra | |
0.24 | - |
D-glucose 1-phosphate | 25°C, pH 5.6 | Brassica nigra | |
0.5 | - |
ATP | 25°C, pH 5.6 | Brassica nigra | |
0.5 | - |
2,3 diphosphoglycerate | 25°C, pH 5.6 | Brassica nigra | |
0.57 | - |
4-nitrophenyl phosphate | 25°C, pH 5.6 | Brassica nigra | |
0.59 | - |
D-gluconate 6-phosphate | 25°C, pH 5.6 | Brassica nigra | |
0.74 | - |
ADP | 25°C, pH 5.6 | Brassica nigra | |
0.82 | - |
phytic acid | 25°C, pH 5.6 | Brassica nigra | |
1.21 | - |
3-phosphoglycerate | 25°C, pH 5.6 | Brassica nigra | |
2.01 | - |
D-glucose 6-phosphate | 25°C, pH 5.6 | Brassica nigra |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.4 fold activation at 4 mM | Brassica nigra | |
Co2+ | 1.4 fold activation at 4 mM | Brassica nigra | |
Mg2+ | 1.7 fold activation at 4 mM | Brassica nigra | |
Mn2+ | 1.4 fold activation at 4 mM | Brassica nigra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Brassica nigra |
56000 | - |
gel filtration | Brassica nigra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + H2O | Brassica nigra | bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation | pyruvate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica nigra | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica nigra |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | petiole suspension cells | Brassica nigra | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity determined for all substrates | Brassica nigra |
380 | - |
purified enzyme, 25°C, pH 5.6 | Brassica nigra |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme, 6 months, stable | Brassica nigra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl-phosphate + H2O | - |
Brassica nigra | 1-naphthol + phosphate | - |
? | |
2,3-diphosphoglycerate + H2O | - |
Brassica nigra | ? | - |
? | |
2-phosphoglycerate + H2O | poor substrate | Brassica nigra | glycerate + phosphate | - |
? | |
3-phosphoglycerate + H2O | - |
Brassica nigra | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Brassica nigra | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Brassica nigra | ? | - |
? | |
ADP-glucose + H2O | poor substrate | Brassica nigra | ? | - |
? | |
ATP + H2O | - |
Brassica nigra | ? | - |
? | |
D-fructose 2,6-diphosphate + H2O | poor substrate | Brassica nigra | ? | - |
? | |
D-gluconate 6-phosphate + H2O | - |
Brassica nigra | D-gluconate + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | - |
Brassica nigra | ? | - |
? | |
D-glucose 6-phosphate + H2O | poor substrate | Brassica nigra | D-glucose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | poor substrate | Brassica nigra | D-ribose + phosphate | - |
? | |
O-phospho-L-threonine + H2O | - |
Brassica nigra | L-threonine + phosphate | - |
? | |
O-phospho-L-tyrosine + H2O | - |
Brassica nigra | L-tyrosine + phosphate | - |
? | |
phosphoenolpyruvate + H2O | - |
Brassica nigra | pyruvate + phosphate | - |
? | |
phosphoenolpyruvate + H2O | bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation | Brassica nigra | pyruvate + phosphate | - |
? | |
phytic acid + H2O | - |
Brassica nigra | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, gel filtration, SDS-PAGE | Brassica nigra |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
4 min, stable | Brassica nigra |
55 | - |
4 min, 20% loss of activity | Brassica nigra |
60 | - |
4 min, 30% loss of activity | Brassica nigra |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Brassica nigra |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | - |
Brassica nigra |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
molybdate | 25°C, pH 5.6 | Brassica nigra | |
0.05 | - |
3-phosphoglycerate | 25°C, pH 5.6 | Brassica nigra | |
0.06 | - |
D-fructose 1,6-bisphosphate | 25°C, pH 5.6 | Brassica nigra | |
0.085 | - |
phosphate | 25°C, pH 5.6 | Brassica nigra |