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Literature summary for 3.1.3.6 extracted from

  • Campbell, T.A.; Zlotnick, G.W.; Neubert, T.A.; Sacci, J.B.; Gottlieb, M.
    Purification and characterization of 3'-nucleotidase/nuclease from promastigotes of Leishmania donovani (1991), Mol. Biochem. Parasitol., 47, 109-117.
    View publication on PubMed
Search for more literature for 3.1.3.6

Application

Application Comment Organism
medicine leishmanial 3'-nucleotidase could become a target for chemotherapeutic attack because an enzyme with similar properties has not been detected in mammalian tissues Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 3'-AMP
-
 Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane surface membrane associated Leishmania donovani 5886
-

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Leishmania donovani

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1827
-
-
 Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Leishmania donovani adenosine + phosphate
-
 ?
3'-GMP + H2O
-
 Leishmania donovani guanosine + phosphate
-
 ?
3'-UMP + H2O
-
 Leishmania donovani uridine + phosphate
-
 ?
adenosine 3',5'-diphosphate + H2O
-
 Leishmania donovani ?
-
 ?

Synonyms

Synonyms Comment Organism
3'-N'ase
-
 Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 the presence of dithiothreitol shifts the optimum to pH 6.0 Leishmania donovani