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Literature summary for 3.1.3.56 extracted from

  • Zhong, R.; Ye, Z.H.
    Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana (2004), Plant Cell Physiol., 45, 1720-1728.
    View publication on PubMed
Search for more literature for 3.1.3.56

Activating Compound

Activating Compound Comment Organism Structure
additional information wounding and abscisic acid induce the enzyme Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
gene 5PTase12, expression of His-tagged full length enzyme in yeast Arabidopsis thaliana
gene 5PTase13, expression of His-tagged full length enzyme in yeast Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, maximal activity at 3 mM Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1D-myo-inositol 1,4,5-trisphosphate + H2O Arabidopsis thaliana specific for 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O80560 gene 5PTase12
-
Arabidopsis thaliana Q9SYK4 gene 5PTase13
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from yeast by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf very low expression of 5PTase12 Arabidopsis thaliana
-
leaf very low expression of 5PTase13 Arabidopsis thaliana
-
additional information expression pattern Arabidopsis thaliana
-
additional information expression pattern, no expression in root Arabidopsis thaliana
-
root low expression of 5PTase12 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-
shoot tip
-
 Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-
stem low expression of 5PTase13 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1D-myo-inositol 1,4,5-trisphosphate + H2O specific for Arabidopsis thaliana 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
1D-myo-inositol 1,4,5-trisphosphate + H2O absolutely specific for Arabidopsis thaliana 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
additional information substrate specificity, no activity with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate, 1D-myo-inositol 1,3,4,5-tetrakisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate, 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1-phosphatidyl-1D-myo-inositol 4-phosphate, and 1-phosphatidyl-1D-myo-inositol 3-phosphate Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme contains a WD-repeat domain and a 5PTase catalytic domain Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5PTase
-
 Arabidopsis thaliana
inositol polyphosphate 5-phosphatase
-
 Arabidopsis thaliana
type II inositol polyphosphate 5-phosphatase
-
 Arabidopsis thaliana