KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
D-myo-inositol 1,4,5-trisphosphate | pH 7.4, 37°C | Bos taurus | |
0.011 | - |
D-myo-Inositol 1,3,4,5-tetrakisphosphate | pH 7.4, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | 85-90% of total activity, can be solubilized by cholate or to lesser extent by 2 M KCl | Bos taurus | 16020 | - |
soluble | 10-15% of total activity | Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration without cholate, type I enzyme | Bos taurus |
48000 | - |
SDS-PAGE, type I enzyme | Bos taurus |
51000 | - |
gel filtration in presence of 0.9% cholate, type I enzyme | Bos taurus |
52000 | - |
gel filtration in presence of 0.9% cholate, type II enzyme | Bos taurus |
115000 | - |
gel filtration without cholate, type II enzyme | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | Bos taurus | - |
D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? | |
D-myo-inositol 1,4,5-trisphosphate + H2O | Bos taurus | regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate | D-myo-inositol 1,4-bisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of membrane bound and soluble enzyme | Bos taurus |
type I enzyme | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
purified type I enzyme, pH 7.4, 37°C | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Bos taurus | D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? | |
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | substrate for type I but not type II enzyme | Bos taurus | D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? | |
D-myo-inositol 1,4,5-trisphosphate + H2O | - |
Bos taurus | D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
D-myo-inositol 1,4,5-trisphosphate + H2O | regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate | Bos taurus | D-myo-inositol 1,4-bisphosphate + phosphate | - |
? |