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Literature summary for 3.1.3.55 extracted from

  • Pato, M.D.; Sutherland, C.; Winder, S.J.; Walsh, M.P.
    Smooth-muscle caldesmon phosphatase is SMP-I, a type 2A protein phosphatase (1993), Biochem. J., 293, 35-41.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 caldesmon pH 7.5, 30°C, caldesmon phosphorylated by PKC cdc2 Meleagris gallopavo
0.0022
-
 caldesmon pH 7.5, 30°C, caldesmon phosphorylated by PKC Meleagris gallopavo
0.0032
-
 caldesmon pH 7.5, 30°C, caldesmon phosphorylated by PKC CaM kinase II Meleagris gallopavo
0.0143
-
 calponin pH 7.5, 30°C, calponin phosphorylated by PKC Meleagris gallopavo

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Meleagris gallopavo 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000 catalytic subunit + x * 55000 + x * 60000, SDS-PAGE Gallus gallus
55000
-
 x * 38000 catalytic subunit + x * 55000 + x * 60000, SDS-PAGE Gallus gallus
60000
-
 x * 38000 catalytic subunit + x * 55000 + x * 60000, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
caldesmon phosphate + H2O Gallus gallus
-
 caldesmon + phosphate
-
 ?
caldesmon phosphate + H2O Meleagris gallopavo
-
 caldesmon + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Meleagris gallopavo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-
gizzard
-
 Meleagris gallopavo
-
muscle
-
 Gallus gallus
-
muscle
-
 Meleagris gallopavo
-

Storage Stability

Storage Stability Organism
-80°C Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caldesmon phosphate + H2O
-
 Gallus gallus caldesmon + phosphate
-
 ?
caldesmon phosphate + H2O
-
 Meleagris gallopavo caldesmon + phosphate
-
 ?
caldesmon phosphate + H2O 3 forms of caldesmon: caldesmon phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaM kinase II), caldesmon phosphorylated by cdc2 kinase, caldesmon phosphorylated by protein kinase C (PKC) Gallus gallus caldesmon + phosphate
-
 ?
caldesmon phosphate + H2O 3 forms of caldesmon: caldesmon phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaM kinase II), caldesmon phosphorylated by cdc2 kinase, caldesmon phosphorylated by protein kinase C (PKC) Meleagris gallopavo caldesmon + phosphate
-
 ?
phosphorylated calponin + H2O
-
 Gallus gallus calponin + phosphate
-
 ?
phosphorylated calponin + H2O
-
 Meleagris gallopavo calponin + phosphate
-
 ?
phosphorylated myosin LC20 + H2O
-
 Gallus gallus myosin LC20 + phosphate
-
 ?
phosphorylated myosin LC20 + H2O
-
 Meleagris gallopavo myosin LC20 + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 38000 catalytic subunit + x * 55000 + x * 60000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gallus gallus
30
-
 assay at Meleagris gallopavo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Gallus gallus
7.5
-
 assay at Meleagris gallopavo