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Literature summary for 3.1.3.54 extracted from

  • Plankert, U.; Purwin, C.; Holzer, H.
    Yeast fructose-2,6-bisphosphate 6-phosphatase is encoded by PHO8, the gene for nonspecific repressible alkaline phosphatase (1991), Eur. J. Biochem., 196, 191-196.
    View publication on PubMed
Search for more literature for 3.1.3.54

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Saccharomyces cerevisiae
Neocuproine i.e. 2,9-dimethyl-1,10-phenanthroline, 0.1 mM Zn2+ stabilizes the activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Saccharomyces cerevisiae 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ dimer contains 1.3 mol copper per mol of subunit Saccharomyces cerevisiae
Zn2+ dimer contains 1.5 mol zinc per mol of subunit Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae
-
 D-fructose 2-phosphate + phosphate
-
 ?
D-fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae M1
-
 D-fructose 2-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae M1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
7000fold, strain M1 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae M1 4-nitrophenol + phosphate
-
 ?
D-fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae D-fructose 2-phosphate + phosphate
-
 ?
D-fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae M1 D-fructose 2-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.7, about 60% loss of activity after 15 min, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
40
-
 pH 7.0, 5 min, stable, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
50
-
 pH 7.0, 5 min, about 30% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae
60
-
 pH 7.0, 5 min, about 70% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 D-fructose 2,6-bisphosphate Saccharomyces cerevisiae
6
-
 with D-fructose 2,6-bisphosphate as substrate Saccharomyces cerevisiae
9
-
 above, 4-nitrophenyl phosphate Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 pH 4: about 25% of activity maximum, pH 7.5: about 20% of activity maximum, fructose 2,6-bisphosphate Saccharomyces cerevisiae