Activating Compound | Comment | Organism | Structure |
---|---|---|---|
poly(L-arginine) | stimulates 1.5-3fold at 0.0036 mg/ml | Bos taurus | |
poly(L-lysine) | stimulates 1.5-3fold at 0.0036 mg/ml | Bos taurus | |
protamine | stimulates 1.5-3fold at 0.0036 mg/ml | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acyl-CoA | inhibition is partially reversed by Mg2+ | Bos taurus | |
ADP | 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
ATP | 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
CDP | 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
CoA | inhibition is not reversed by Mg2+ | Bos taurus | |
CTP | 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
GDP | 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
GTP | 50% inhibition at 60 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
guanosine 5'-(beta,gamma-imido)triphosphate | - |
Bos taurus | |
heparin | 50% inhibition at 0.002 mg/ml, partially reversed by Mg2+ | Bos taurus | |
additional information | little inhibitory effect: AMP; little inhibitory effect: GMP; little inhibitory effect: NAD+; little inhibitory effect: NADH; little inhibitory effect: UMP | Bos taurus | |
UDP | 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ | Bos taurus | |
UTP | 50% inhibition at 100 mM; inhibition is completely reversed by Mg2+ | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | active in absence of divalent cation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
gel filtration, under conditions of high dilution, low MW form | Bos taurus |
460000 | - |
gel filtration | Bos taurus |
460000 | - |
high MW form | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O | Bos taurus | - |
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a high and a low MW form | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, partially purified enzyme stable for several weeks | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O | - |
Bos taurus | [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.3 | - |
Bos taurus |