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Literature summary for 3.1.3.51 extracted from
- A dolichyl phosphate-cleaving acid phosphatase from Tetrahymena pyriformis (1979), Biochim. Biophys. Acta, 575, 431-438.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethoxy formic anhydride | 10 mM, 67% inhibition of dolichyl phosphate hydrolysis, no inhibition of p-nitrophenyl phosphate cleavage | Tetrahymena pyriformis |  |
| Tartrate | inhibits hydrolysis of p-nitrophenyl phosphate, no inhibition of the hydrolysis of dolichyl phosphate | Tetrahymena pyriformis | |
| vanadium oxide | inhibits hydrolysis of p-nitrophenyl phosphate and dolichyl phosphate | Tetrahymena pyriformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | hydrolysis of dolichyl phosphate requires Mg2+ | Tetrahymena pyriformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Tetrahymena pyriformis | the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis | ? | - |
? | |
| additional information | Tetrahymena pyriformis WH14 | the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
- |
- |
| Tetrahymena pyriformis WH14 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate + H2O | - |
Tetrahymena pyriformis | dolichol + phosphate | - |
? | |
| dolichyl phosphate + H2O | - |
Tetrahymena pyriformis WH14 | dolichol + phosphate | - |
? | |
| additional information | the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis | Tetrahymena pyriformis | ? | - |
? | |
| additional information | the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis | Tetrahymena pyriformis WH14 | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Tetrahymena pyriformis | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Tetrahymena pyriformis WH14 | p-nitrophenol + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
reaction rate increases up to | Tetrahymena pyriformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, 75% loss of activity | Tetrahymena pyriformis |
| 60 | - |
10 min, complete loss of activity | Tetrahymena pyriformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Tetrahymena pyriformis |
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Release 2023.1 (January 2023)
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