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Literature summary for 3.1.3.5 extracted from

  • Lai, K.M.; Wong, P.C.L.
    A comparison of the properties of 5'-nucleotidase purified from the cytosolic and synaptic plasma membrane fractions of rat forebrain (1991), Int. J. Biochem., 23, 1123-1130.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-(alpha,beta-methylene)diphosphate
-
Rattus norvegicus
ADP
-
Rattus norvegicus
ATP
-
Rattus norvegicus
Co2+ inhibition of cytosolic and membrane-bound enzyme Rattus norvegicus
Cu2+ inhibition of cytosolic and membrane-bound enzyme Rattus norvegicus
EDTA inhibition of cytosolic and membrane-bound enzyme Rattus norvegicus
Mn2+ inhibition of cytosolic and membrane-bound enzyme Rattus norvegicus
Zn2+ inhibition of cytosolic and membrane-bound enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
5'-GMP synaptic membrane-bound Rattus norvegicus
0.048
-
5'-TMP synaptic membrane-bound Rattus norvegicus
0.056
-
5'-IMP synaptic membrane-bound Rattus norvegicus
0.074
-
5'-TMP brain cytosol Rattus norvegicus
0.083
-
5'-AMP synaptic membrane-bound Rattus norvegicus
0.09
-
5'-GMP brain cytosol Rattus norvegicus
0.09
-
5'-UMP synaptic membrane-bound Rattus norvegicus
0.14
-
5'-CMP synaptic membrane-bound Rattus norvegicus
0.18
-
5'-UMP brain cytosol Rattus norvegicus
0.19
-
5'-CMP brain cytosol Rattus norvegicus
0.2
-
5'-IMP brain cytosol Rattus norvegicus
0.27
-
5'-AMP brain cytosol Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus 5737
-
plasma membrane
-
Rattus norvegicus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
x * 72000, synaptic plasma membrane, SDS-PAGE Rattus norvegicus
131000
-
x * 131000, brain cytosol, SDS-PAGE Rattus norvegicus
268000
-
brain cytosol, gel filtration Rattus norvegicus
286000
-
enzyme from synaptic plasma membrane, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification cytosolic and membrane-bound enzyme from brain are both glycoproteins Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
brain cytosol and synaptic plasma membrane Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.48
-
cytosolic enzyme from brain Rattus norvegicus
60.5
-
synaptic plasma membrane Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
Rattus norvegicus adenosine + phosphate
-
?
5'-CMP + H2O
-
Rattus norvegicus cytidine + phosphate
-
?
5'-dTMP + H2O
-
Rattus norvegicus deoxythymidine + phosphate
-
?
5'-GMP + H2O
-
Rattus norvegicus guanosine + phosphate
-
?
5'-IMP + H2O
-
Rattus norvegicus inosine + phosphate
-
?
5'-UMP + H2O
-
Rattus norvegicus uridine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 72000, synaptic plasma membrane, SDS-PAGE Rattus norvegicus
? x * 131000, brain cytosol, SDS-PAGE Rattus norvegicus