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Literature summary for 3.1.3.5 extracted from

  • Camici, M.; Fini, C.; Ipata, P.L.
    Isolation and kinetic properties of 5'-nucleotidase from guinea-pig skeletal muscle (1985), Biochim. Biophys. Acta, 840, 6-12.
    View publication on PubMed
Search for more literature for 3.1.3.5

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Cavia porcellus
ATP
-
 Cavia porcellus
Ca2+
-
 Cavia porcellus
concanavalin A
-
 Cavia porcellus
EDTA
-
 Cavia porcellus
Mg2+
-
 Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 5'-AMP
-
 Cavia porcellus
0.05
-
 5'-dAMP
-
 Cavia porcellus
0.059
-
 5'-GMP
-
 Cavia porcellus
0.1
-
 5'-IMP
-
 Cavia porcellus
0.11
-
 5'-UMP
-
 Cavia porcellus
0.36
-
 5'-CMP
-
 Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Cavia porcellus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Cavia porcellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, SDS-PAGE Cavia porcellus
185000
-
 gel filtration Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.4
-
-
 Cavia porcellus

Storage Stability

Storage Stability Organism
-20°C Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Cavia porcellus adenosine + phosphate
-
 ?
5'-CMP + H2O 125% of the activity with 5'-AMP Cavia porcellus cytidine + phosphate
-
 ?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Cavia porcellus deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O 22% of the activity with 5'-AMP Cavia porcellus deoxyadenosine + phosphate
-
 ?
5'-GMP + H2O 117% of the activity with 5'-AMP Cavia porcellus guanosine + phosphate
-
 ?
5'-IMP + H2O 92% of the activity with 5'-AMP Cavia porcellus inosine + phosphate
-
 ?
5'-UMP + H2O 117% of the activity with 5'-AMP Cavia porcellus uridine + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Cavia porcellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min stable Cavia porcellus
56
-
 15 min, 20% loss of activity Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Cavia porcellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Cavia porcellus