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Literature summary for 3.1.3.5 extracted from

  • Trummal, K.; Samel, M.; Aaspollu, A.; Tonismaegi, K.; Titma, T.; Subbi, J.; Siigur, J.; Siigur, E.
    5'-Nucleotidase from Vipera lebetina venom (2015), Toxicon, 93, 155-163 .
    View publication on PubMed
Search for more literature for 3.1.3.5

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 89.5% residual activity at 5 mM Macrovipera lebetina
Cu2+ 3.9% residual activity at 5 mM Macrovipera lebetina
EDTA
-
 Macrovipera lebetina
Fe3+ 3.3% residual activity at 5 mM Macrovipera lebetina
Mg2+ 80.9% residual activity at 5 mM Macrovipera lebetina
vanillic acid
-
 Macrovipera lebetina
Zn2+ 2.1% residual activity at 5 mM Macrovipera lebetina

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme activity is enhanced by Mn2+ (170% activity at 5 mM) Macrovipera lebetina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Macrovipera lebetina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-AMP + H2O Macrovipera lebetina
-
 adenosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Macrovipera lebetina W8EFS0
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography, CM52 cellulose column chromatography, Superdex 200 gel filtration, and Sephadex G-100 gel filtration Macrovipera lebetina

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Macrovipera lebetina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Macrovipera lebetina adenosine + phosphate
-
 ?
5'-AMP + H2O best substrate Macrovipera lebetina adenosine + phosphate
-
 ?
5'-GMP + H2O
-
 Macrovipera lebetina guanosine + phosphate
-
 ?
ADP + H2O
-
 Macrovipera lebetina 5'-AMP + phosphate
-
 ?
ATP + H2O lowest activity Macrovipera lebetina ADP + phosphate
-
 ?
additional information no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP Macrovipera lebetina ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Macrovipera lebetina

Synonyms

Synonyms Comment Organism
5'-NT
-
 Macrovipera lebetina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 the enzyme is stable from 10-37°C. The activity of the enzyme decreases after heating at 37°C, at 50°C approximately two third of the activity is left, at 60°C the activity is almost entirely lost Macrovipera lebetina

General Information

General Information Comment Organism
physiological function the enzyme strongly inhibits ADP- or collagen-induced platelet aggregation Macrovipera lebetina