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Literature summary for 3.1.3.5 extracted from

  • Zheng, L.; Khemlani, A.; Lorenz, N.; Loh, J.M.; Langley, R.J.; Proft, T.
    Streptococcal 5'-nucleotidase A (S5nA), a novel Streptococcus pyogenes virulence factor that facilitates immune evasion (2015), J. Biol. Chem., 290, 31126-31137 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
F414A the enzymatic activity of the mutant is significantly reduced compared to the wild type enzyme Streptococcus pyogenes
H100A inactive Streptococcus pyogenes
N99A inactive Streptococcus pyogenes
R392A the mutant shows very weak activity compared to the wild type enzyme Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ about 30% enzymatic activity is achieved at 0.05 mM, and the activity drops further to about 15% for 0.5 mM or higher concentrations of Zn2+ Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1683
-
 5'-AMP wild type enzyme, at pH 7.4 and 37°C Streptococcus pyogenes
0.275
-
 5'-AMP mutant enzyme F414A, at pH 7.4 and 37°C Streptococcus pyogenes
0.8
-
 5'-AMP Km above 0.8 mM, mutant enzyme R392A, at pH 7.4 and 37°C Streptococcus pyogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is active in the presence of Ca2+, 2 mM used in assay conditions Streptococcus pyogenes
Mg2+ the enzyme is active in the presence of Mg2+, 2 mM used in assay conditions Streptococcus pyogenes
Mn2+ the enzyme is active in the presence of Mn2+ Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes Q9A0A2 SF370
-
Streptococcus pyogenes ATCC 700294 Q9A0A2 SF370
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-iminodiacetic acid Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O best substrate Streptococcus pyogenes adenosine + phosphate
-
 ?
5'-AMP + H2O best substrate Streptococcus pyogenes ATCC 700294 adenosine + phosphate
-
 ?
5'-CMP + H2O
-
 Streptococcus pyogenes cytidine + phosphate
-
 ?
5'-CMP + H2O
-
 Streptococcus pyogenes ATCC 700294 cytidine + phosphate
-
 ?
5'-dAMP + H2O
-
 Streptococcus pyogenes deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O
-
 Streptococcus pyogenes ATCC 700294 deoxyadenosine + phosphate
-
 ?
5'-GMP + H2O
-
 Streptococcus pyogenes guanosine + phosphate
-
 ?
5'-GMP + H2O
-
 Streptococcus pyogenes ATCC 700294 guanosine + phosphate
-
 ?
5'-TMP + H2O
-
 Streptococcus pyogenes thymidine + phosphate
-
 ?
ADP + H2O
-
 Streptococcus pyogenes 5'-AMP + phosphate
-
 ?
additional information no activity with ATP Streptococcus pyogenes ?
-
 ?
additional information no activity with ATP Streptococcus pyogenes ATCC 700294 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes
? x * 69870, recombinant enzyme, calculated from amino acid sequence Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
5'-nucleotidase A
-
 Streptococcus pyogenes
5nA
-
 Streptococcus pyogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 47
-
 Streptococcus pyogenes

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 the optimal enzymatic activity is between pH 5.0 and pH 6.5. The activity decreases at higher pH but is still at about 25% at pH 9.0 Streptococcus pyogenes

General Information

General Information Comment Organism
physiological function the enzyme is a Streptococcus pyogenes virulence factor that facilitates immune evasion from the host Streptococcus pyogenes