Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Crystallization (Comment) | Organism |
---|---|
X-ray structure of catalytically inactivate variant D51N of murine cN-III with substrate uridine 5'-monophosphate at 1.74 A resolution. The aromatic ring of a nitrogenous base of the substrate nucleotide is stabilized by parallel pi-stacking interactions with conserved aromatic rings of Trp113 and His68. The nitrogenous base is further stabilized by T-shaped stacking with the conserved aromatic ring of Tyr114, as well as by polar contacts with side chains of Thr66 and Ser117. Two water molecules help to stabilize the nucleotide binding by bridging it to protein residues Asp72 and His68 via hydrogen bonds. Glu96 is responsible for recognition of ribose ring via two hydrogen bonds | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D51N | complete loss of activity | Mus musculus |
E96S | significant decrease in catalytic ability | Mus musculus |
H68A | significant decrease in catalytic ability | Mus musculus |
N69G | slightly higher Km compared to the wild?type | Mus musculus |
S117G | no significant changes compared to wild-type | Mus musculus |
T66G | no significant changes compared to wild-type | Mus musculus |
W113A | significant decrease in catalytic ability | Mus musculus |
Y114A | significant decrease in catalytic ability | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
5'-UMP | wild-type, pH 7.5, 37°C | Mus musculus | |
1.1 | - |
5'-UMP | mutant S117G, pH 7.5, 37°C | Mus musculus | |
1.1 | - |
5'-UMP | mutant T66G, pH 7.5, 37°C | Mus musculus | |
2.1 | - |
5'-UMP | mutant N69G, pH 7.5, 37°C | Mus musculus | |
2.3 | - |
5'-UMP | mutant Y114A, pH 7.5, 37°C | Mus musculus | |
3.2 | - |
5'-UMP | mutant H68A, pH 7.5, 37°C | Mus musculus | |
4.8 | - |
5'-UMP | mutant E96S, pH 7.5, 37°C | Mus musculus | |
29 | - |
5'-UMP | mutant W11A, pH 7.5, 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9D020 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-UMP + H2O | - |
Mus musculus | uridine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cN-III | - |
Mus musculus |
NT5C3A | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
5'-UMP | mutant H68A, pH 7.5, 37°C | Mus musculus | |
0.14 | - |
5'-UMP | mutant Y114A, pH 7.5, 37°C | Mus musculus | |
0.29 | - |
5'-UMP | mutant W11A, pH 7.5, 37°C | Mus musculus | |
0.38 | - |
5'-UMP | mutant E96S, pH 7.5, 37°C | Mus musculus | |
1.1 | - |
5'-UMP | mutant N69G, pH 7.5, 37°C | Mus musculus | |
1.2 | - |
5'-UMP | wild-type, pH 7.5, 37°C | Mus musculus | |
1.7 | - |
5'-UMP | mutant S117G, pH 7.5, 37°C | Mus musculus | |
1.9 | - |
5'-UMP | mutant T66G, pH 7.5, 37°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5'-UMP | mutant W11A, pH 7.5, 37°C | Mus musculus | |
0.037 | - |
5'-UMP | mutant H68A, pH 7.5, 37°C | Mus musculus | |
0.06 | - |
5'-UMP | mutant Y114A, pH 7.5, 37°C | Mus musculus | |
0.08 | - |
5'-UMP | mutant E96S, pH 7.5, 37°C | Mus musculus | |
0.55 | - |
5'-UMP | mutant N69G, pH 7.5, 37°C | Mus musculus | |
1.1 | - |
5'-UMP | wild-type, pH 7.5, 37°C | Mus musculus | |
1.5 | - |
5'-UMP | mutant S117G, pH 7.5, 37°C | Mus musculus | |
1.7 | - |
5'-UMP | mutant T66G, pH 7.5, 37°C | Mus musculus |