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Literature summary for 3.1.3.5 extracted from
- Crystal structure of a soluble form of human CD73 with ecto-5-nucleotidase activity (2012), ChemBioChem, 13, 2384-2391.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| soluble form of human soluble CD73 at 2.2 A resolution, a truncated form of CD73, residues 27549, that retains ecto-5'-nucleotidase activity. Structure reveals a conserved loop that is directly involved in the dimer-dimer interaction showing that the two subunits of the dimer are not linked by disulfide bridges | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | further inhibition of the residual activity in EDTA-treated enzyme | Homo sapiens |  |
| EDTA | 2 mM, 5% residual activity | Homo sapiens | |
| Fe2+ | further inhibition of the residual activity in EDTA-treated enzyme | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
5'-AMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.0065 | - |
5'-GMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.0071 | - |
5'-IMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.008 | - |
5'-TMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.0316 | - |
5'-UMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.0564 | - |
5'-CMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 0.114 | - |
5'-dAMP | 37°C, pH not specified in the publication | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 30% recovery of initial activity with EDTA-treated enzyme | Homo sapiens | |
| additional information | no effect: Mg2+ | Homo sapiens | |
| additional information | divalent metal ion is required | Homo sapiens | |
| Zn2+ | 60% recovery of initial activity with EDTA-treated enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, truncated form of enzyme | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6NZX3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sequence contains four potential gycosylation sites, Asn53, Asn311, Asn333 and Asn403. The enzyme is decorated with a variety of oligosaccharide structures | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Homo sapiens | adenosine + phosphate | - |
? | |
| 5'-CMP + H2O | - |
Homo sapiens | cytidine + phosphate | - |
? | |
| 5'-dAMP + H2O | - |
Homo sapiens | deoxyadenosine + phosphate | - |
? | |
| 5'-GMP + H2O | - |
Homo sapiens | guanosine + phosphate | - |
? | |
| 5'-IMP + H2O | - |
Homo sapiens | inosine + phosphate | - |
? | |
| 5'-TMP + H2O | - |
Homo sapiens | thymidine + phosphate | - |
? | |
| 5'-UMP + H2O | - |
Homo sapiens | uridine + phosphate | - |
? | |
| additional information | poor substrate: beta-nicotinamide mononucleotide | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, truncated form of enzyme | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CD73 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40 | - |
5'-TMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 104 | - |
5'-IMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 132 | - |
5'-GMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 174 | - |
5'-dAMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 187 | - |
5'-AMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 202 | - |
5'-CMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 289 | - |
5'-UMP | 37°C, pH not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1500 | - |
5'-dAMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 3600 | - |
5'-CMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 5000 | - |
5'-TMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 9100 | - |
5'-UMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 14700 | - |
5'-IMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 20300 | - |
5'-GMP | 37°C, pH not specified in the publication | Homo sapiens | |
| 48800 | - |
5'-AMP | 37°C, pH not specified in the publication | Homo sapiens | |
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