Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-bisphosphoglycerate | - |
Bos taurus | |
ADP | - |
Bos taurus | |
ATP | - |
Bos taurus | |
diadenosine polyphosphate | - |
Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant enzymes | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
D396A | site-directed mutagenesis, the mutant is only activable by 2,3-bisphosphoglycerate | Bos taurus |
F127E | site-directed mutagenesis | Bos taurus |
F36R | site-directed mutagenesis, inactive mutant | Bos taurus |
G319D | site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme | Bos taurus |
H428D | site-directed mutagenesis | Bos taurus |
I152D | site-directed mutagenesis | Bos taurus |
K311A | site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme | Bos taurus |
M436W | site-directed mutagenesis | Bos taurus |
N154D | site-directed mutagenesis, the mutant shows a decrease in the value of K50 for Mg2+ compared to the wild-type enzyme | Bos taurus |
R144E | site-directed mutagenesis | Bos taurus |
T56V | site-directed mutagenesis | Bos taurus |
Y115A | site-directed mutagenesis, the mutant behaves similar to the wild-type enzyme | Bos taurus |
Y55G | site-directed mutagenesis | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant K311A | Bos taurus | |
0.05 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant G319D | Bos taurus | |
0.06 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant Y115A | Bos taurus | |
0.1 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant wild-type enzyme and mutant D396A | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-IMP + H2O | Bos taurus | - |
inosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | O46411 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-IMP + H2O | - |
Bos taurus | inosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the active tetramer is formed by interaction between two identical dimers via interface B: this interface contains 28 aminoacid residues, of which 8 make hydrogen bonds | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
cN-II | - |
Bos taurus |
cytosolic 5'-nucleotidase | - |
Bos taurus |