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Literature summary for 3.1.3.5 extracted from

  • Wu, J.Z.; Larson, G.; Walker, H.; Shim, J.H.; Hong, Z.
    Phosphorylation of ribavirin and viramidine by adenosine kinase and cytosolic 5'-nucleotidase II: Implications for ribavirin metabolism in erythrocytes (2005), Antimicrob. Agents Chemother., 49, 2164-2171.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2,3-bisphosphoglycerate
-
Homo sapiens
ATP
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for viramidine is above 0.5 mM Homo sapiens
0.024
-
ribavirin presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 Homo sapiens
0.047
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
0.088
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, phosphate-buffered saline, pH 7.4 Homo sapiens
0.095
-
ribavirin presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens
1.8
-
Inosine presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
2.17
-
Inosine presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P49902 isoform cytosolic 5'-nucleotidase II, expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + 5'-IMP
-
Homo sapiens 5'-IMP + inosine
-
?
ribavirin + 5'-IMP better substrate than viramidine Homo sapiens ?
-
?
viramidine + 5'-IMP worse substrate than ribavirin Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
ribavirin presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 Homo sapiens
0.027
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
0.067
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, PBS-buffer, pH 7.4 Homo sapiens
0.15
-
ribavirin presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens
19
-
Inosine presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
22
-
Inosine presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens