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Literature summary for 3.1.3.5 extracted from

  • Fini, C.; Talamo, F.; Cherri, S.; Coli, M.; Floridi, A.; Ferrara, L.; Scaloni, A.
    Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma (2003), Biochem. J., 372, 443-451.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.5

Activating Compound

Activating Compound Comment Organism Structure
alpha,beta-methylene-adenosine 5'-diphosphate inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 Bos taurus
ATP inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4 Bos taurus

Application

Application Comment Organism
medicine target for a number of therapeutic drugs since increased levels of the enzyme correlate with various disease states Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 dCMP isoenzyme 5'-NT-3 Bos taurus
0.04
-
 dCMP isoenzyme 5'-NT-2 Bos taurus
0.05
-
 dCMP isoenzyme 5'-NT-1 Bos taurus
0.06
-
 5'-AMP isoenzyme 5'-NT-2 and 5'-NT-4 Bos taurus
0.06
-
 5'-IMP isoenzyme 5'-NT-3 Bos taurus
0.06
-
 dCMP isoenzyme 5'-NT-4 Bos taurus
0.07
-
 5'-IMP isoenzyme 5'-NT-2 and 5'-NT-4 Bos taurus
0.07
-
 5'-GMP isoenzyme 5'-NT-4 Bos taurus
0.09
-
 5'-GMP isoenzyme 5'-NT-2 Bos taurus
0.11
-
 5'-AMP isoenzyme 5'-NT-1 and 5'-NT-3 Bos taurus
0.15
-
 5'-IMP isoenzyme 5'-NT-1 Bos taurus
0.16
-
 5'-GMP isoenzyme 5'-NT-3 Bos taurus
0.22
-
 5'-GMP isoenzyme 5'-NT-1 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane glycosylphosphatidyl-inositol-anchored Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66200
-
 x * 66200, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein differential glycosylation, especially in the oligosaccharides linked to Asn403, an increase in sialylated glycans, together with a decrease in high-mannose structures is observed arising from 5'-NT-1 to 5'-NT-4 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
300
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Bos taurus adenosine + phosphate
-
 ?
5'-GMP + H2O
-
 Bos taurus guanosine + phosphate
-
 ?
5'-IMP + H2O
-
 Bos taurus inosine + phosphate
-
 ?
dCMP + H2O
-
 Bos taurus deoxycytidine + phosphate
-
 ?
additional information significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site Bos taurus ?
-
 ?
additional information the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66200, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
5'-NT-1
-
 Bos taurus
5'-NT-2
-
 Bos taurus
5'-NT-3
-
 Bos taurus
5'-NT-4
-
 Bos taurus
ecto-5'-NT
-
 Bos taurus
ecto-5'-nucleotidase
-
 Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus isoenzyme 5'-NT-4
-
 6.1
Bos taurus isoenzyme 5'-NT-3
-
 6.2
Bos taurus isoenzyme 5'-NT-2 6.8 6.3
Bos taurus isoenzyme 5'-NT-1
-
 6.5