Cloned (Comment) | Organism |
---|---|
gene PTPRD, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Homo sapiens |
gene PTPRR, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl phosphate | substrate inhibition at higher concentrations; substrate inhibition at higher concentrations | Homo sapiens | |
additional information | IPTG and lactose inhibit the sugar hydrolase activity of the enzyme PTPRdelta | Homo sapiens | |
phosphate | inhibits the phosphatase activity, but not the sugar hydrolase activity; inhibits the phosphatase activity, but not the sugar hydrolase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.64 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
1.04 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
37 | - |
bis(4-nitrophenyl) phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | receptor protein tyrosine phosphatases (PTPRs) are a family of cell surface receptor proteins | Homo sapiens | 9986 | - |
membrane | receptor protein tyrosine phosphatases (PTPRs) are a family of cell surface receptor proteins | Homo sapiens | 16020 | - |
additional information | receptor protein tyrosine phosphatase subtype delta has a cell adhesion extracellular domain and two cytoplasmic protein tyrosine phosphatase (PTP) domains | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens | |
Mg2+ | activates the sugar hydrolase activity, but not the phosphatase activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Homo sapiens | - |
[a protein]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23468 | - |
- |
Homo sapiens | Q15256 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate | the phosphatase domain of PTPRs catalyze a two-step phosphate monoester hydrolysis reaction through a highly conserved sequence motif (H/V)CX5R(S/T) with a nucleophilic cysteine | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | PTPRdelta is predominantly expressed in the brain | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
bis(4-nitrophenyl) phosphate + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | receptor protein tyrosine phosphatase isoform delta (PTPRdelta) displays substrate promiscuity by hydrolyzing the diester bond while showing a high-level of discrimination in its primary monoesterase substrate preference. The phosphatase domain of receptor protein tyrosine phosphatases catalyzes the hydrolysis of glycosidic (COC) bonds apart from its primary activity of cleaving phosphomonoester (COP) bond. This is important because the hydrolysis of COC bond in beta-galactosides and COP bond in phosphomonoesters require different functional groups and different mechanism of cleavage. No activity with adenosine monophosphate, inosine monophosphate, guanosine monophosphate, nicotinamide adenine dinucleotide phosphate, and pyridoxal 5'-phosphate, also no activity with tris-(4-nitrophenyl) phosphate, tris-(4-nitrobenzyl) phosphate, tetrakis-(4-nitrophenyl) diphosphate, NADP+, and peptide H-Gly-PNA. The enzyme shows low activity with several nitrophenyl glycosides cleaving the glycosidic bond. Substrate specificity, overview. No phosphocysteinyl hydrolysis of isozyme PTPRdelta | Homo sapiens | ? | - |
? | |
additional information | receptor protein tyrosine phosphatase isoform delta (PTPRdelta) shows catalytic and substrate promiscuity. The phosphatase domain of receptor protein tyrosine phosphatases catalyzes the hydrolysis of glycosidic (COC) bonds apart from its primary activity of cleaving phosphomonoester (COP) bond. This is important because the hydrolysis of COC bond in beta-galactosides and COP bond in phosphomonoesters require different functional groups and different mechanism of cleavage. No activity with adenosine monophosphate, inosine monophosphate, guanosine monophosphate, nicotinamide adenine dinucleotide phosphate, and pyridoxal 5'-phosphate, also no activity with tris-(4-nitrophenyl) phosphate, tris-(4-nitrobenzyl) phosphate, tetrakis-(4-nitrophenyl) diphosphate, NADP+, and peptide H-Gly-PNA. The enzyme shows low activity with several nitrophenyl glycosides cleaving the glycosidic bond. Substrate specificity, overview. Isozyme PD-PTPRomega shows rate-limiting phosphocysteinyl hydrolysis leading to a biphasic time-dependence of substrate to product conversion | Homo sapiens | ? | - |
? | |
O-phospho-L-tyrosine + H2O | - |
Homo sapiens | tyrosine + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Homo sapiens | [a protein]-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34267, recombinant His-tagged enzyme, mass spectrometry | Homo sapiens |
More | receptor protein tyrosine phosphatase subtype delta has a cell adhesion extracellular domain and two cytoplasmic protein tyrosine phosphatase (PTP) domains | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Ch-1PTPase | UniProt | Homo sapiens |
NC-PTPCOM1 | UniProt | Homo sapiens |
PCPTP1 | UniProt | Homo sapiens |
protein tyrosine phosphatase | - |
Homo sapiens |
PTP | - |
Homo sapiens |
PTPRD | - |
Homo sapiens |
PTPRdelta | - |
Homo sapiens |
PTPRomega | - |
Homo sapiens |
PTPRQ | - |
Homo sapiens |
PTPRR | - |
Homo sapiens |
receptor protein tyrosine phosphatase isoform delta | - |
Homo sapiens |
receptor protein tyrosine phosphatase isoform omega | - |
Homo sapiens |
receptor-type tyrosine-protein phosphatase R | UniProt | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
0.033 | - |
bis(4-nitrophenyl) phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
0.207 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
phosphatase activity | Homo sapiens |
7.3 | - |
assay at | Homo sapiens |
7.3 | - |
sugar hydrolase activity | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | receptor protein tyrosine phosphatases (PTPRs) are a family of cell surface receptor proteins that antagonize tyrosine kinase signaling | Homo sapiens |
physiological function | the primary catalytic activity involves the hydrolysis of a phosphomonoester bond (C-O-P) with high catalytic efficiency, while the secondary activity is the hydrolysis of a glycosidic bond (C-O-C) with poorer catalytic efficiency. This enzyme also displays substrate promiscuity by hydrolyzing diester bonds while being highly discriminative for its monoester substrates. Additional distinct sugar hydrolase and diesterase activity for the phosphatase domain of PTPRdelta and its homologue PTPRomega | Homo sapiens |
physiological function | the primary catalytic activity involves the hydrolysis of a phosphomonoester bond (C-O-P) with high catalytic efficiency, while the secondary activity is the hydrolysis of a glycosidic bond (C-O-C) with poorer catalytic efficiency. This enzyme also displays substrate promiscuity by hydrolyzing diester bonds while being highly discriminative for its monoester substrates. Additional distinct sugar hydrolase and diesterase activity for the phosphatase domain of PTPRdelta and its homologue PTPRomega. PTPRdelta is known to be involved in the guidance and termination of motor neurons during embryonic development, PTPRdelta is essential for the organization of neural circuits | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
bis(4-nitrophenyl) phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
0.042 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens | |
0.199 | - |
4-nitrophenyl phosphate | pH 7.3, 22°C, recombinant enzyme | Homo sapiens |