Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
x * 17500, calculated from amino acid sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Mycobacterium tuberculosis | - |
[a protein]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIA1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at Tyr128 and Tyr129 is catalyzed by the kinase PtkA | Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Mycobacterium tuberculosis | 4-nitrophenol + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Mycobacterium tuberculosis | [a protein]-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17500, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
LMW-PTP | - |
Mycobacterium tuberculosis |
low molecular weight protein-tyrosine phosphatase A | - |
Mycobacterium tuberculosis |
MPtpA | - |
Mycobacterium tuberculosis |
protein-tyrosine phosphatase | - |
Mycobacterium tuberculosis |
PTP | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | low molecular weight protein-tyrosine phosphatase A is a key virulence factor of Mycobacterium tuberculosis, the enzyme is essential for infection of host macrophages | Mycobacterium tuberculosis |