Cloned (Comment) | Organism |
---|---|
gene ptp-H2, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged wild-type and mutant PTP-H2 in Escherichia coli cells as non-fusion or thioredoxinfusion proteins | Bracoviriform demolitoris |
Protein Variants | Comment | Organism |
---|---|---|
C236S | site-directed mutagenesis, mutant PTP-H2C236S, the non-fusion mutant has almost no enzymatic activity, while the thioredoxin-fusion form has low levels of activity | Bracoviriform demolitoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bracoviriform demolitoris | |
heparin | - |
Bracoviriform demolitoris | |
additional information | the enzyme activity is diminished by increasing acetate buffer ionic strength, but PTP-H2 is unaffected by tetramisole and levamisole, as well as by okadaic acid, sodium fluoride and sodium citrate | Bracoviriform demolitoris | |
vanadate | - |
Bracoviriform demolitoris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | increases the enzyme activity | Bracoviriform demolitoris | |
additional information | divalent cations Zn2+, Ca2+, Mg2+, and Mn2+ have little or no negative effect on activity | Bracoviriform demolitoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bracoviriform demolitoris | - |
gene ptp-H2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant PTP-H2 from Escherichia coli cells by nickel affinity chromatography | Bracoviriform demolitoris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | localization in host hemocytes following infection by Microplitis demolitor bracovirus | Bracoviriform demolitoris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ENDpYINASL + H2O | - |
Bracoviriform demolitoris | ENDYINASL + phosphate | - |
? | |
additional information | no activity with DADEpYLIPQQG, poor activity with 4-nitrophenyl phosphate | Bracoviriform demolitoris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTP-H2 | - |
Bracoviriform demolitoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Bracoviriform demolitoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Bracoviriform demolitoris |
General Information | Comment | Organism |
---|---|---|
additional information | PTP-H2 expression is restricted to hemocytes in Microplitis demolitor bracovirus -infected hosts | Bracoviriform demolitoris |