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Literature summary for 3.1.3.48 extracted from

  • Abdo, M.; Liu, S.; Zhou, B.; Walls, C.D.; Wu, L.; Knapp, S.; Zhang, Z.Y.
    Seleninate in place of phosphate: irreversible inhibition of protein tyrosine phosphatases (2008), J. Am. Chem. Soc., 130, 13196-13197.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C403S a catalytically inactive mutant YopH Yersinia sp.

Inhibitors

Inhibitors Comment Organism Structure
(4-[(2S)-2-[(tert-butoxycarbonyl)amino]-3-methoxy-3-oxopropyl]phenyl)methaneseleninic acid inactivates the PTPs, e.g. YopH, by covalent modification, binds at the active site Cys403, kinetics, overview Yersinia sp.
methyl 4-[(benzylseleninyl)methyl]-N-(tert-butoxycarbonyl)-L-phenylalaninate
-
Yersinia sp.
methyl N-(tert-butoxycarbonyl)-4-(hydroxymethyl)-L-phenylalaninate
-
Yersinia sp.
methyl N-(tert-butoxycarbonyl)-4-[(5R)-5-(methoxycarbonyl)-9,9-dimethyl-7-oxo-8-oxa-3-thia-2-selena-6-azadec-1-yl]-L-phenylalaninate
-
Yersinia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33512
-
x * 33512, YopH PTP domain, residues 163-468, electrospray ion trap mass spectrometry Yersinia sp.

Organism

Organism UniProt Comment Textmining
Yersinia sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in the YopH PTP catalytic mechanism, the active site Cys403 sits at the bottom of the pTyr-binding pocket, i.e., the active site, such that its Sgamma atom is poised 3 A from the phosphorus atom of the substrate ready for nucleophilic attack Yersinia sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 33512, YopH PTP domain, residues 163-468, electrospray ion trap mass spectrometry Yersinia sp.

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Yersinia sp.
YopH
-
Yersinia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Yersinia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Yersinia sp.