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Literature summary for 3.1.3.46 extracted from
- Purification and characterization of fructose-2,6-bisphosphatase, a substrate-specific cytosolic enzyme from leaves (1989), J. Biol. Chem., 264, 5540-5544.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Spinacia oleracea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Spinacia oleracea | |
| fructose 6-phosphate | - |
Spinacia oleracea | |
| MgCl2 | - |
Spinacia oleracea | |
| phosphate | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
fructose 2,6-bisphosphate | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Spinacia oleracea |
| 76200 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | Spinacia oleracea | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 10000fold | Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | - |
Spinacia oleracea | fructose 6-phosphate + phosphate | - |
? | |
| fructose 2,6-bisphosphate + H2O | - |
Spinacia oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
pretreatment with MgATP2-, form 1 | Spinacia oleracea |
| 7.3 | - |
- |
Spinacia oleracea |
| 7.6 | - |
pretreatment with MgATP2-, form 2 | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | - |
Spinacia oleracea |
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