Any feedback?
Literature summary for 3.1.3.46 extracted from
- Regulation of the regulatory enzyme, 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase (2004), Adv. Enzyme Regul., 44, 123-154.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| formate | activates, interacts with Glu327 | Rattus norvegicus |  |
| additional information | the hepatic enzyme expression is stimulated at transcriptional level by both insulin and glucocorticoids | Homo sapiens | |
| additional information | the hepatic enzyme expression is stimulated at transcriptional level by both insulin and glucocorticoids, overview | Rattus norvegicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of the liver enzyme | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E327A | site-directed mutagenesis, inactive mutant, can be rescued by formate at pH 7.0, overview | Rattus norvegicus |
| H258A | site-directed mutagenesis, mutation of the strong nucleophile His258 reduces activity compared to the wild-type enzyme | Rattus norvegicus |
| H446A | site-directed mutagenesis, the mutation perturbs significantly and specificially the resonances at the catalytic site assigned to His258 and His392 in His-specific multinuclear NMR study, interaction of residue 446 with R397 is disrupted, comparison to the wild-type enzyme, overview | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | inhibits the cardiac enzyme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O | Homo sapiens | beta-D-fructose 2,6-bisphosphate synthesis is catalyzed by the second enzyme activity 6-phosphofructo-2-kinase, EC 2.7.1.105 | D-fructose 6-phosphate + phosphate | - |
? | |
| beta-D-fructose 2,6-bisphosphate + H2O | Rattus norvegicus | beta-D-fructose 2,6-bisphosphate synthesis is catalyzed by the second enzyme activity 6-phosphofructo-2-kinase, EC 2.7.1.105 | D-fructose 6-phosphate + phosphate | - |
? | |
| additional information | Rattus norvegicus | the enzyme is important in degradation of the biolocical factor beta-D-fructose 2,6-bisphosphate, the bifunctional PFK-2/FDPase-2 shows a metabolic switch to change between the two separate activities, involved in glycolysis and gluconeogenesis, metabolic regulation overview | ? | - |
? | |
| additional information | Homo sapiens | the enzyme is important in degradation of the biological factor beta-D-fructose 2,6-bisphosphate, the bifunctional PFK-2/FDPase-2 shows a metabolic switch to change between the two separate activities, metabolic regulation overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
bifunctional enzyme | - |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | catalytic phosphorylation of His residues is essential, overview | Rattus norvegicus |
| phosphoprotein | the enzyme contains a regulatory Ser32 phosphorylation site in each regulatory region, catalytic phosphorylation of His residues is essential, overview | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | catalytic phosphorylation of His residues is essential, overview | Homo sapiens | |
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | catalytic site structure and interactions involving residues H258, E327, H392, R397, and H446, detailed molecular mechanism and regulation in wild-type and mutant enzymes, overview, catalytic phosphorylation of His residues is essential, overview | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| liver | expression is regulated at transcriptional level by both insulin and glucocorticoids | Homo sapiens | - |
| liver | expression is regulated at transcriptional level by both insulin and glucocorticoids | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O | beta-D-fructose 2,6-bisphosphate synthesis is catalyzed by the second enzyme activity 6-phosphofructo-2-kinase, EC 2.7.1.105 | Homo sapiens | D-fructose 6-phosphate + phosphate | - |
? | |
| beta-D-fructose 2,6-bisphosphate + H2O | beta-D-fructose 2,6-bisphosphate synthesis is catalyzed by the second enzyme activity 6-phosphofructo-2-kinase, EC 2.7.1.105 | Rattus norvegicus | D-fructose 6-phosphate + phosphate | - |
? | |
| additional information | the enzyme is important in degradation of the biolocical factor beta-D-fructose 2,6-bisphosphate, the bifunctional PFK-2/FDPase-2 shows a metabolic switch to change between the two separate activities, involved in glycolysis and gluconeogenesis, metabolic regulation overview | Rattus norvegicus | ? | - |
? | |
| additional information | the enzyme is important in degradation of the biological factor beta-D-fructose 2,6-bisphosphate, the bifunctional PFK-2/FDPase-2 shows a metabolic switch to change between the two separate activities, metabolic regulation overview | Homo sapiens | ? | - |
? | |
| additional information | catalytic cycle of the catalytic domain of fructose-2 6-biphosphatase, overview | Rattus norvegicus | ? | - |
? | |
| additional information | catalytic cycle of the catalytic domain of fructose-2 6-biphosphatase, overview, the bifunctional PFK-2/FDPase-2 shows a metabolic switch to change between the two activities, involved in glycolysis and gluconeogenesis, regulation overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the bifunctional liver PFK-2/FDPase-2 has an N-terminal and a C-terminal regulatory region flanking the catalytic core domain harbouring both enzyme active sites, overview, the two activities are physically coupled via the tertiary and quarternary structure | Homo sapiens |
| More | the regulatory region His446 is linked with the catalytic site His258 and His392 via Arg397, overview | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase | - |
Homo sapiens |
| 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase | - |
Rattus norvegicus |
| More | bifunctional enzyme, cf. EC 2.7.1.105 | Homo sapiens |
| More | bifunctional enzyme, cf. EC 2.7.1.105 | Rattus norvegicus |
| PFK-2/FDPase-2 | - |
Homo sapiens |
| PFK-2/FDPase-2 | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
