Literature summary for 3.1.3.46 extracted from

Villadsen, D.; Nielsen, T.H.
N-terminal truncation affects the kinetics and structure of fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase from Arabidopsis thaliana (2001), Biochem. J., 359, 591-597.

Search for more literature for 3.1.3.46

Protein Variants

Protein Variants	Comment	Organism
additional information	N-terminal truncation mutants, behave as monomers, show a decrease in the kinase/phosphatase ration by 4-fold, role of N-terminus for subunit assembly and kinetic properties	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
6-phosphogluconate	-	Arabidopsis thaliana
D-fructose-1,6-bisphosphate	-	Arabidopsis thaliana
D-fructose-6-phosphate	-	Arabidopsis thaliana
D-glucose-1,6-bisphosphate	-	Arabidopsis thaliana
additional information	not inhibitory: diphosphate	Arabidopsis thaliana
phosphate	-	Arabidopsis thaliana
pyruvate	-	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000028	-	D-fructose-1,6-bisphosphate	pH 6.0, 25°C	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
83000	-	4 * 83000, deduced from gene sequence	Arabidopsis thaliana
370000	-	gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9MB58	bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, recombinant enzyme, expressed in yeast	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 2,6-bisphosphate + H2O	-	Arabidopsis thaliana	D-fructose 6-phosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 83000, deduced from gene sequence	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
42	-	10 min, inactivation, phosphate protects	Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.08	-	6-phosphogluconate	-	Arabidopsis thaliana
0.08	-	D-fructose-1,6-bisphosphate	-	Arabidopsis thaliana
0.1	-	D-fructose-6-phosphate	-	Arabidopsis thaliana
0.5	-	phosphate	-	Arabidopsis thaliana
0.85	-	D-glucose-1,6-bisphosphate	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)