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Literature summary for 3.1.3.45 extracted from

  • Daughtry, K.D.; Huang, H.; Malashkevich, V.; Patskovsky, Y.; Liu, W.; Ramagopal, U.; Sauder, J.M.; Burley, S.K.; Almo, S.C.; Dunaway-Mariano, D.; Allen, K.N.
    Structural basis for the divergence of substrate specificity and biological function within HAD phosphatases in lipopolysaccharide and sialic acid biosynthesis (2013), Biochemistry, 52, 5372-5386.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacteroides thetaiotaomicron
expressed in Escherichia coli BL21(DE3) cells Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C Bacteroides thetaiotaomicron
enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
0.0009
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
0.16
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
1.6
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacteroides thetaiotaomicron
Mg2+ required Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O Bacteroides thetaiotaomicron best substrate ? + phosphate
-
 ?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O Haemophilus influenzae best substrate ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
-
-
-
Haemophilus influenzae P45314
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Bacteroides thetaiotaomicron
TALON metal affinity resin column chromatography Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
-
 Bacteroides thetaiotaomicron ? + phosphate
-
 ?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
-
 Haemophilus influenzae ? + phosphate
-
 ?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O best substrate Bacteroides thetaiotaomicron ? + phosphate
-
 ?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O best substrate Haemophilus influenzae ? + phosphate
-
 ?
KDN 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
-
 ?
KDN 9-phosphate + H2O
-
 Haemophilus influenzae 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Bacteroides thetaiotaomicron
dimer x-ray crystallography Haemophilus influenzae

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
-
 Bacteroides thetaiotaomicron
2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
-
 Haemophilus influenzae
KDO8P phosphatase
-
 Bacteroides thetaiotaomicron
KDO8P phosphatase
-
 Haemophilus influenzae
KDO8PP
-
 Bacteroides thetaiotaomicron
KDO8PP
-
 Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.021
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae
0.061
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
0.78
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
1.5
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.013
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae
0.069
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
4.8
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
15
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron