Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli | |
Cd2+ | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
3-deoxy-D-manno-octulosonate 8-phosphate | pH 7.0, 37°C, presence of 1 mM Mg2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, little stimulation | Escherichia coli | |
Co2+ | 1 mM, stimulation about 9fold | Escherichia coli | |
Mg2+ | 1 mM, stimulation about 9fold | Escherichia coli | |
Mn2+ | 1 mM, little stimulation | Escherichia coli | |
additional information | divalent metal cofactor required | Escherichia coli | |
Zn2+ | 1 mM, little stimulation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19900 | - |
4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
23000 | - |
4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
89000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-manno-octulosonate 8-phosphate + H2O | high specificity | Escherichia coli | 3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
additional information | last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | 4.8 | 4.7 |