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Literature summary for 3.1.3.41 extracted from

  • Sato, V.S.; Galdiano Junior, R.F.; Rodrigues, G.R.; Lemos, E.G.; Pizauro Junior, J.M.
    Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae (2016), J. Microbiol., 54, 106-113 .
    View publication on PubMed
Search for more literature for 3.1.3.41

Inhibitors

Inhibitors Comment Organism Structure
arsenate competitive. 10 mM, 39% residual activity Enterobacter asburiae
additional information not inhibitory: EDTA, calcium, copper, cobalt, levamisole, and 4-hydroxymercuribenzoate Enterobacter asburiae
phosphate competitive. 10 mM, 58% residual activity Enterobacter asburiae
vanadate 10 mM, 63% residual activity Enterobacter asburiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 4-nitrophenyl phosphate K0.5 value, Hill coefficient 1.2, pH 3.5, 37°C Enterobacter asburiae
0.084
-
 diphosphate K0.5 value, Hill coefficient 2.3, pH 3.5, 37°C Enterobacter asburiae
0.11
-
 ATP K0.5 value, Hill coefficient 1.6, pH 3.5, 37°C Enterobacter asburiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Enterobacter asburiae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM, 120% of initial activity Enterobacter asburiae

Organism

Organism UniProt Comment Textmining
Enterobacter asburiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Enterobacter asburiae 4-nitrophenol + phosphate
-
 ?
ATP + H2O
-
 Enterobacter asburiae ADP + phosphate
-
 ?
diphosphate + H2O
-
 Enterobacter asburiae 2 phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Enterobacter asburiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 arsenate pH 3.5, 37°C Enterobacter asburiae
1.8
-
 phosphate pH 3.5, 37°C Enterobacter asburiae

Expression

Organism Comment Expression
Enterobacter asburiae expression is induced by presence of phosphate up