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Literature summary for 3.1.3.4 extracted from

  • Guo, A.; Cai, J.; Luo, X.; Zhang, S.; Hou, J.; Li, H.; Cai, X.
    Cloning and characterization of three Eimeria tenella lipid phosphate phosphatases (2015), PLoS ONE, 10, e122736 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged EtLPP1 in HEK293 cells produces immunoreactive proteins with variable molecular sizes, suggesting the presence of multiple forms of enzyme Eimeria tenella

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is insensitive to N-ethylmaleimide; the enzyme is insensitive to N-ethylmaleimide; the enzyme is insensitive to N-ethylmaleimide Eimeria tenella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 ceramide-1-phosphate pH 6.5, 37°C Eimeria tenella
0.028
-
 sphingosine-1-phosphate pH 6.5, 37°C Eimeria tenella
0.046
-
 lysophosphatidate pH 6.5, 37°C Eimeria tenella
0.052
-
 ceramide-1-phosphate pH 6.5, 37°C Eimeria tenella
0.083
-
 lysophosphatidate pH 6.5, 37°C Eimeria tenella
0.179
-
 lysophosphatidate pH 6.5, 37°C Eimeria tenella
0.237
-
 sphingosine-1-phosphate pH 6.5, 37°C Eimeria tenella
0.26
-
 sphingosine-1-phosphate pH 6.5, 37°C Eimeria tenella
0.284
-
 phosphatidate pH 6.5, 37°C Eimeria tenella
0.355
-
 phosphatidate pH 6.5, 37°C Eimeria tenella
0.369
-
 phosphatidate pH 6.5, 37°C Eimeria tenella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 and 60000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3 Eimeria tenella
40000
-
 and 42000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1 Eimeria tenella
40000
-
 and 70000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2 Eimeria tenella
42000
-
 and 40000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP1 Eimeria tenella
60000
-
 and 37000 Da and 110000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3 Eimeria tenella
70000
-
 and 40000 Da and 120000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2 Eimeria tenella
110000
-
 and 60000 Da and 37000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP3 Eimeria tenella
120000
-
 and 40000 da and 70000 Da, SDS-PAGE, multiple forms of enzyme probably representing the non-glycoslylated and glycosylated forms of EtLPP2 Eimeria tenella

Organism

Organism UniProt Comment Textmining
Eimeria tenella A0A0A7H6F4
-
-
Eimeria tenella A0A0A7H9C0
-
-
Eimeria tenella A0A0A7H9U0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is partially N-glycosylated Eimeria tenella

Source Tissue

Source Tissue Comment Organism Textmining
sporozoite particularly high expression levels of the three lipid phosphate phosphatase (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage Eimeria tenella
-
sporozoite particularly high expression levels of the three lipid phosphate phosphatases (EtLPP) genes are detected in the sporozoite stage of the Eimeria tenella life cycle, suggesting that their encoded proteins might play an important biological function in the sporozoite stage Eimeria tenella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ceramide-1-phosphate + H2O
-
 Eimeria tenella ceramide + phosphate
-
 ?
lysophosphatidate + H2O
-
 Eimeria tenella monoacylglycerol + phosphate
-
 ?
phosphatidate + H2O
-
 Eimeria tenella diacylglycerol + phosphate
-
 ?
sphingosine-1-phosphate + H2O
-
 Eimeria tenella sphingosine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
EtLPP1
-
 Eimeria tenella
EtLPP2
-
 Eimeria tenella
EtLPP3
-
 Eimeria tenella

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Eimeria tenella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Eimeria tenella