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Literature summary for 3.1.3.4 extracted from

  • Comba, S.; Menendez-Bravo, S.; Arabolaza, A.; Gramajo, H.
    Identification and physiological characterization of phosphatidic acid phosphatase enzymes involved in triacylglycerol biosynthesis in Streptomyces coelicolor (2013), Microb. Cell Fact., 12, 0009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Streptomyces coelicolor 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, SDS-PAGE Streptomyces coelicolor
40000
-
x * 40000, SDS-PAGE Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9EWX3 isoform Ippbeta
-
Streptomyces coelicolor Q9K3P6 isoform Ippalpha
-
Streptomyces coelicolor ATCC BAA-471 Q9EWX3 isoform Ippbeta
-
Streptomyces coelicolor ATCC BAA-471 Q9K3P6 isoform Ippalpha
-

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Streptomyces coelicolor
? x * 40000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
Ippalpha
-
Streptomyces coelicolor
Ippbeta
-
Streptomyces coelicolor
SCO1102
-
Streptomyces coelicolor
SCO1753
-
Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function expression of the gene in yeast complements the temperature-sensitive growth phenotype of the phosphatidic acid phosphatase deficient strain GHY58. In Streptomyces coelicolor, disruption of either isoform lppalpha or lppbeta has no effect on triacylglycerol accumulation. The simultaneous mutation of both genes provokes a drastic reduction in de novo triacylglycerol biosynthesis as well as in total triacylglycerol content. Overexpression of Lppalpha and Lppbeta in the wild type strain of Streptomyces coelicolor leads to a significant increase in triacylglycerol production. Membrane proteins isolated from an Escherichia coli strain expressing Lppalpha and Lppbeta display a considerable increase in phosphatidic acid phosphatase activity compared with the control strain Streptomyces coelicolor