Protein Variants | Comment | Organism |
---|---|---|
S110A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
S114A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
S168A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
S602A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
S744A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
S748A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
T723A | phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32567 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at sites Ser110, Ser114, Ser168, Ser602, Thr723, Ser744, and Ser748 is managed by protein kinase-cyclin complex Pho85p-Pho80p. The phosphorylation of recombinant isoform Pah1p is time- and dose-dependent and dependent on the concentrations of ATP and Pah1p. Phosphorylation reduces the catalytic efficiency 6fold and reduces 3fold its interaction with liposomes. Alanine mutations of the seven sites ablates the inhibitory effect of phosphorylation. Loss of Pho85p-Pho80p phosphorylation reduces Pah1p abundance. Lack of Nem1p-Spo7p, the phosphatase complex that dephosphorylates Pah1p at the nuclear/endoplasmic reticulum membrane, stabilizes Pah1p abundance | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
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Synonyms | Comment | Organism |
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Pah1p | - |
Saccharomyces cerevisiae |